Culture Development White Papers and Case Studies

  1. Application Note: Perfusion Culture Of Cell-Seeded 3D Scaffolds For Tissue Engineering 5/19/2011
    Since oxygen has a low solubility in culture medium it must be supplied to cells within 3D scaffolds via constant perfusion of the medium. The oxygen content of the medium before and after having passed by the cells can then help to determine whether the flow rate applied is sufficient. By R. Santoro, B. Pippenger, I. Martin and D. Wendt, Department of Surgery and Research, University Hospital, Basel, Switzerland
  2. Two-Step Purification Of Monoclonal IgG1 From CHO Cell Culture Supernatant 4/13/2011

    The increasing demand for monoclonal antibodies (MAb) as biopharmaceuticals has promoted the development of cell cultures with increased expression levels. As a consequence, the demand for more effi cient purifi cation processes has increased. This application note describes a two-step process for MAb purifi cation based on MabSelect SuRe™ and Capto™ adhere, a strong multimodal anion exchanger. 

  3. The Design And Testing Of A Continuous Effluent Sterilization System For Liquid Waste 12/22/2010
    The disposal of microbiologically contaminated or potentially contaminated liquid waste is an important consideration for research facilities and other installations. This report describes a new continuous effluent decontamination system that allows for the continuous collection, thermal sterilization, and cooldown of wastewater prior to discarding from the facility. By STERIS Corporation
  4. pH Regulation For A Liver Cell Bioreactor 4/27/2010
    An important aspect of efficient liver cell bioreactors is the automated regulation of physio-chemical culture parameters. By Marco Decker
  5. Application Note: pH Regulation For A Liver Cell Bioreactor 4/27/2010
    An important aspect of efficient liver cell bioreactors is the automated regulation of physiochemical culture parameters. The present study describes the development of the first noninvasive pH regulation device for a perfusion bioreactor. By Marco Decker
  6. Process Monitoring in Suspension-Adapted CHO Cell Cultures 4/27/2010
    On-line detection of oxygen and pH at a small-scale can be used as a basis for latter cultivation in large-scale bioreactors to detect optimal growth conditions. Contamination-free on-line monitoring of culture parameters is not only important for scaling up production in process development, but it could also be valuable for other applications such as tissue engineering, stem cell research, and toxicological tests. By PreSens Precision Sensing GmbH
  7. Application Note: Process Monitoring In Suspension-Adapted CHO Cell Cultures 4/27/2010
    On-line detection of oxygen and pH at a small-scale can be used as a basis for latter cultivation in large-scale bioreactors to detect optimal growth conditions. Contamination-free on-line monitoring of culture parameters is not only important for scaling up production in process development, but it could also be valuable for other applications such as tissue engineering, stem cell research, and toxicological tests. By PreSens Precision Sensing GmbH
  8. What Are Mycoplasmas? 12/8/2009

    They are considered to be the smallest free-living organisms capable of self replication with a genome, and have become one of the most prevalent and serious microbial contaminants within cell culture systems used in research and industry today.

  9. Application Note: Automated Washing Of Magnetic Bead QuantiGene® Plex 2.0 Assays 9/24/2009
    The characterization of gene expression through the quantitation of mRNA species from experimental samples is a commonly used technique in biomedical research today. The QuantiGene® Plex 2.0 assay kit from Affymetrix offers a means to quantitate multiple mRNA species simultaneously from a variety of different sample types. This technology, which uses ferrite beads as a solid substrate for the reactions requires a magnetic field for bead retention during the wash steps. Here we describe the use of the ELx405™ Magnetic Bead Washer to automate the wash steps of this assay technology. By Paul Held, Principal Scientist, Applications Dept., BioTek Instruments, Inc.
  10. Automated Washing Of Magnetic Bead QuantiGene® Plex 2.0 Assays 9/24/2009
    The characterization of gene expression through the quantitation of mRNA species from experimental samples is a commonly used technique in biomedical research today. The QuantiGene® Plex 2.0 assay kit from Affymetrix offers a means to quantitate multiple mRNA species simultaneously from a variety of different sample types. This technology, which uses ferrite beads as a solid substrate for the reactions requires a magnetic field for bead retention during the wash steps. Here we describe the use of the ELx405™ Magnetic Bead Washer to automate the wash steps of this assay technology. By Paul Held, Principal Scientist, Applications Dept., BioTek Instruments, Inc.