Culture Development White Papers and Case Studies
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Propagation Of Influenza Virus In Vero Cells
2/9/2012
The aim of this work was to establish a process to produce influenza virus in Vero cell culture using single-use equipment. The end result would be a fast and simple process enabling scale-up and adaptation to industrial production. The following parameters were evaluated: medium composition, cell detachment, medium supplements, agitation conditions, and infection conditions.
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Employee-Driven Change Management Takes Copernicus Group IRB Completely Paperless
12/29/2011
Copernicus Group IRB (CGIRB), with assistance from Sitrof and employee-driven change management, was able to transform from an entirely paper-reliant company with millions of pages of legacy documentation into a completely digital paperless organization in less than two years.
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Biomanufacturing Flexibility
12/22/2011
This white paper from BIO-G looks at how to create flexible, agile manufacturing facilities to respond to the current glut of biomanufacturing capacity and industry consolidation. Future proofing these facilities for higher titers and multiple product lines is also explored.
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Perfusion And Single-Use Bioreactors: A Partnership For Scale Up
11/3/2011
Perfusion has emerged as a viable technology for the commercial production of biopharmaceuticals. Perfusion solutions can offer very cost-efficient scale up solutions, driving down operating costs due to the smaller equipment and facilities needed as compared to batch or fed-batch systems.
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Considerations For The Development Of Stable Cell Lines From Parental Lines
9/30/2011
Quality standards are often difficult to properly anticipate across today’s global regulatory environments. In recent years, quality expectations have increased due to public demand and to government regulation. This article explores some important considerations when determining quality standards for parental cell lines.
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Chromatography Column Protection With 3M Purification LifeASSURE™ PLA Series Membrane Filters
9/14/2011
Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.
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Development Of A Partly Controllable System At Shake Flask Scale
9/2/2011
The main objective of this study was to develop an easy to handle and partly controllable process cultivation system at shake flask scale.
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Automated Washing Of Multiplex Bead Based Assays For The Luminex MAGPIX® Reader System
8/22/2011
Luminex xMAP® technology provides the means to measure multiple analytes simultaneously from the same sample. By Paul Held Ph.D., Laboratory Manager, Applications Dept., BioTek Instruments, Inc.
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Application Note: Perfusion Culture Of Cell-Seeded 3D Scaffolds For Tissue Engineering
5/19/2011
Since oxygen has a low solubility in culture medium it must be supplied to cells within 3D scaffolds via constant perfusion of the medium. The oxygen content of the medium before and after having passed by the cells can then help to determine whether the flow rate applied is sufficient. By R. Santoro, B. Pippenger, I. Martin and D. Wendt, Department of Surgery and Research, University Hospital, Basel, Switzerland
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Two-Step Purification Of Monoclonal IgG1 From CHO Cell Culture Supernatant
4/13/2011
The increasing demand for monoclonal antibodies (MAb) as biopharmaceuticals has promoted the development of cell cultures with increased expression levels. As a consequence, the demand for more effi cient purifi cation processes has increased. This application note describes a two-step process for MAb purifi cation based on MabSelect SuRe™ and Capto™ adhere, a strong multimodal anion exchanger.