Application Note: pH Regulation For A Liver Cell Bioreactor

By Marco Decker

An important aspect of efficient liver cell bioreactors is the automated regulation of physio-chemical culture parameters. The present study describes the development of the first non-invasive pH regulation device for a perfusion bioreactor. The high performance of the system is based on a chemical-optical flow-through cell for pH detection and its combination with precision mass-flow controllers for gas. The new controller allows long time stable and contamination free on-line pH regulation in complex bioreactor systems - an important technical contribution for future clinical applications.