Bioprocessing Application Notes & Case Studies

31. End-User Evaluation Of 30 L And 300 L HyPerforma Single-Use Fermentors And Scale-Down Model 3/1/2021

    In moving processes from bench to pilot and ultimately production fermentors, it is critical to have a reliable scaledown model. Scale-down models can vary depending on the media, strain, and process conditions. In this case, a customer wanted a scale-down model of the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) in order to establish what fermentation processes could be scaled into the S.U.F., in particular for the production of plasmid DNA.

32. Production Of Therapeutic Plasmid DNA In Single-Use Fermentors 3/1/2021

    As producers move toward single-use production to meet demand for gene-targeting biotherapeutics, we review three procedures for production of therapeutic plasmid DNA in single-use fermentors.

33. Faster And Easier Charge Heterogeneity Analysis With The iCE3 3/1/2021

    This paper reviews usability improvements now available for the iCE3 system that allow for greater speed and improved ease of use; simplifies sample preparation, while saving precious time and eliminates a major source of cathodic drift.

34. Liposome Testing 2/26/2021

    Liposomes are spherical engineered particles made of phospholipids used in the pharmaceutical and cosmetic industries. The size and surface charge of liposomes are important characteristics which require measurement and monitoring. Dynamic light scattering (DLS) is the most common analytical technique used for measuring the size of submicron liposomes. Single particle optical sizing (SPOS) is used to measure the size of liposomes greater than one micron. The Entegris Nicomp® DLS system and AccuSizer® SPOS system are used in laboratories around the world for the accurate measurement of the size and charge (zeta potential) of liposomes.

35. A Fixation Protocol Compatible With R&D Systems Luminex Assays Enabling Analysis With Infectious Samples 2/22/2021

    A common way to inactivate infectious material is to use formaldehyde fixation. Confirmation of microorganism inactivation is accomplished using viability testing with broth or growth media for bacteria and plaque assays for viruses. Unfortunately, chemical fixation using formaldehyde has been shown to alter cytokines and chemokines in such a way that an immunoassay would not properly quantify biological samples. To address this issue, we decided to test the hypothesis that R&D Systems Luminex Assays are compatible with standard fixation protocols.

36. Residual Protein A For Bioprocessing Applications 2/19/2021

    Most therapeutic monoclonal antibodies (mAbs) are isolated from cell culture media using Protein A chromatography. During purification, Protein A can leach from the solid phase, ending up in the purified product. This can be problematic as regulatory guidelines dictate that detection and reduction of residual Protein A are necessary to obviate potential immunogenic consequences in vivo.  ProteinSimple’s Wes™ provides a powerful solution by bringing immunoassay into a capillary electrophoresis format.

37. Automated Particle Analysis Of Viscous Samples With MFI And The Bot1 Autosampler 2/19/2021

    Micro-Flow Imaging™ (MFI) easily detects particle size and morphology on a wide range of particle contaminants. And when you add the Bot 1 Autosampler to your MFI 5000 Series system, you’ve got the automated go-to method of choice for particle analysis today. In this application note, we studied how the Bot1 handled viscous samples, and found that with just a few simple optimization steps, you can be running viscous samples in no time at all.

38. Protein Expression Heterogeneity With Milo, The First Single-Cell Western System 2/18/2021

    Every cell is unique, making cell-to-cell heterogeneity important in many areas of biomedicine including cancer pathogenesis, immuno-oncology and regenerative medicine. More and more high-profile publications are using single-cell analysis techniques to reveal variability in cellular response to a drug or stimulus. Milo, the first and only Single-Cell Western platform out there, lets you do Western blotting at the single-cell level.

39. A Custom, Multiplex Human Coronavirus Serological Assay 2/18/2021

    R&D Systems, a Bio-Techne brand, has developed a novel custom Luminex serological assay hat distinguishes between coronavirus subtypes. Using our high-quality recombinant proteins, this antigen-down assay saves time and precious sample by multiplexing many coronavirus subtypes simultaneously in each sample.

40. AcroPrep™ 24-well Filter Plates With Various Pore Size For Ultrafiltration, Microfiltration And Macrofiltration 2/17/2021

    Many techniques in Life Science research rely on efficient sample preparation methods. Concentration and purification of samples are key steps in the workflow that can greatly affect the outcome of the experimental results. Pall has increased the range of AcroPrep 24-well filter plates offered to help streamline membrane selection and provide effective solutions for multiple sample preparation needs. This document reports the data generated by ultrafiltration of proteins of known size with AcroPrep 24-well filter plates.