Duo Digital Dispenser™ For Single-Cell And Low-Input Proteomics

The Duo Digital Dispenser™ offers a streamlined solution for single-cell and low-input proteomics workflows, combining ultra-low volume reagent dispensing with precise cell isolation. This application note highlights Duo’s ability to maintain high single-cell occupancy (91% ± 3.5%) and multi-cell accuracy across hundreds of wells, outperforming its predecessor in throughput and resolution. The system supports both label-free and TMT-multiplexed mass spectrometry, enabling flexible experimental design. Sample preparation is fast—under two hours—and compatible with standard autosamplers. Mass spectrometry results confirmed stable expression of housekeeping proteins across 1–10 cell samples, with no cross-contamination observed. Duo’s software features, including well randomization, help eliminate bias and enhance data reliability.

For researchers aiming to miniaturize workflows without compromising sensitivity or integrity, see how this platform offers a compelling advantage.