Label-Free Analysis Of Multiple Spheroids In A Matrix

This application note presents a streamlined workflow for generating and analyzing multiple tumor spheroids embedded in Matrigel using label-free imaging and AI-based segmentation. By leveraging automated z-stack imaging with the Spark® Cyto multimode reader, researchers can overcome common challenges in 3D cell culture, such as poor image resolution and labor-intensive data processing. The system enables real-time monitoring of spheroid development across varying cell densities and supports flexible experimental setups with open kinetic runs. Quantitative analysis is enhanced through automated image tiling, projection, and segmentation, with validation against fluorescence-based methods showing high correlation and minimal deviation. The integrated software tools also allow for easy reanalysis, gating, and export of data, making it suitable for heterogeneous samples.

Explore the full application note to see how label-free imaging can simplify and scale your 3D cell culture workflows.