Culture Development White Papers and Case Studies
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Application Note: Automated Washing Of Magnetic Bead QuantiGene® Plex 2.0 Assays
9/24/2009
The characterization of gene expression through the quantitation of mRNA species from experimental samples is a commonly used technique in biomedical research today. The QuantiGene® Plex 2.0 assay kit from Affymetrix offers a means to quantitate multiple mRNA species simultaneously from a variety of different sample types. This technology, which uses ferrite beads as a solid substrate for the reactions requires a magnetic field for bead retention during the wash steps. Here we describe the use of the ELx405™ Magnetic Bead Washer to automate the wash steps of this assay technology. By Paul Held, Principal Scientist, Applications Dept., BioTek Instruments, Inc.
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Automated Washing Of Magnetic Bead QuantiGene® Plex 2.0 Assays
9/24/2009
The characterization of gene expression through the quantitation of mRNA species from experimental samples is a commonly used technique in biomedical research today. The QuantiGene® Plex 2.0 assay kit from Affymetrix offers a means to quantitate multiple mRNA species simultaneously from a variety of different sample types. This technology, which uses ferrite beads as a solid substrate for the reactions requires a magnetic field for bead retention during the wash steps. Here we describe the use of the ELx405™ Magnetic Bead Washer to automate the wash steps of this assay technology. By Paul Held, Principal Scientist, Applications Dept., BioTek Instruments, Inc.
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Application Note: Optimizing Performance Of The Membrane-Free, Oris™ Cell Migration Assay For High Throughput Screening Using The BioTek Synergy™ HT Multi-Mode Microplate Reader
4/13/2009
Cell migration is a fundamental activity intrinsic to development and maintenance of homeostasis in processes such as wound healing, neovascularization and the workings of the immune system. The failure of cells to migrate, or the movement of invasive cells into inappropriate locations, is central to many disease processes such as dysregulated wound healing and cancer. By BioTek Instruments, Inc.
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Application Note: Using BioTek's Synergy HT Reader To Measure Reactive Oxygen Species (ROS) Generation In Stimulated Cells
12/21/2008
Reactive Oxygen Species (ROS) is a phrase used to describe a number of reactive molecules and free radicals derived from molecular oxygen. Reactive oxygen species were originally thought to be only released by phagocytic cells as part of their role in host defense. Recent work has demonstrated that ROS have a role in cell signaling, including; apoptosis; gene expression; and the activation of cell signaling cascades. By Paul Held Ph. D, BioTek Instruments, Inc., Kheng Newick, Graduate Student, University of Vermont
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White Paper: Performance Evaluation Of An Automated Bioreactor Sampling System For Mammalian Cell Cultivation
11/4/2008
Two common goals of biotherapeutics development are reduction in timelines and increased process understanding. An automated bioreactor sampling system developed by Groton Biosystems has proven its capacity to address those two seemingly conflicting needs for the industry. Due to the extreme length and stringent sterility requirement of mammalian cell cultivation, sampling automation of mammalian bioreactors remains an unmet need. By Groton Biosystems
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Application Note: Rapid Isolation Of Antigen-Specific Clones From Hybridoma Fusions
10/15/2008
Screening a hybridoma fusion and then subcloning candidate hybrids are time-consuming elements of the monoclonal antibody discovery process. The Genetix ClonePix FL provides a powerful way to find and collect antigen-specific clones in a rapid one-step process. The technology works by automatically screening the entire fusion in situ and isolating only antigen-specific clones with the desired immunoglobulin isotype. If the fusion has many antigen-positive clones, then ClonePix FL can selectively collect only the best secretors. By Genetix Limited
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Case Study: Manufacture Of A Cell-Based Biologic For The Clinic
7/15/2008
This case study describes the steps and processes required for the Manufacture Of A Cell-Based Biologic For The Clinic as recently completed by FLorida Biologix, a CGMP Manufacturer located in Alachua, Florida
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Automated Cross Flow Filtration For Process Development, Filter Screening, And Process Optimization At Small Scales
9/13/2007
This application note focuses on microfiltration (pore sizes between 0.1 µm and 1 µm). Microfiltration is ideally suited for separation of cultured cells from the growth medium (broth), as well as for sterilization, and contaminant and particle removal in numerous biopharmaceutical processes
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Technical Note: CellMaker PLUS For The Culture Of CHO-S Cells
8/24/2007
In recent years there has been a significant increase in the demand for mammalian cell culture procedures in the development and production of biopharmaceuticals. This report describes the use of a Cellexus HybridBagTM single-use bioreactor bag for the fed -batch scale-up of a standard CHO-S (Invitrogen) cell culture in defined CHO-S media (Gibco) in the CellMaker PLUSTM system
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White Paper: MUC-1 Tumor Antigen
8/6/2007
This scientific brief presents important information scientists require relating to the antigen’s clinical relevance, molecular form, measurement and purity determinations by ratio and analysis.
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