Bioprocessing Application Notes & Case Studies

1. ACQUITY And XBridge Premier Protein SEC 250 Å Columns: A New Benchmark In Inert SEC Column Design 7/5/2022

   We demonstrate the performance of a new column technology designed to provide a holistic solution to the problems of undesired secondary interactions in SEC.

2. Performance Stability Of CORTECS 2.7 μm Columns Following Repeated Injections Of Precipitated Plasma 7/1/2022

   We review a column made using optimally packed solid-core particles. These columns are designed to tolerate repeated injections of minimally processed samples, while maintaining assay performance.

3. High-Throughput Multi-Capillary SDS Gel Electrophoresis Of Proteins 7/1/2022

   Discover how a multi-capillary system can be used to accelerate development of sensitive, high-throughput analytical methods, while potentially decreasing time to market for biologics.

4. Improvements In Recovery, Reproducibility, And Matrix Effects With A Novel Solid Phase Extraction Sorbent 6/30/2022

   In this study we review a successful next generation reversed  solid-phase extraction (SPE) product that produces cleaner extracts with simpler protocols and faster processing time.

5. Selecting A Reversed-Phase Column For The Peptide Mapping Analysis Of A Biotherapeutic Protein 6/30/2022

   In this study we review reversed-phase (RP) column selection considerations for peptide mapping applied to the routine characterization and analysis of biotherapeutic proteins.

6. BEH C₁₈ Batch-To-Batch Robustness For The Analysis Of Rosuvastatin And Impurities 6/30/2022

   The main goal of this study is to investigate the long-term batch-to-batch reproducibility of 10 different lots of XBridge BEH C₁₈ Columns. Discover the findings of this study. 

7. A Look At Column Reproducibility: Analysis Of Endogenous Steroid Hormones In Serum 6/30/2022

   Having reproducible columns is critical for the success of batch to batch testing of stationary phases, as well as ensuring lifelong performance for the assay.

8. Increasing Chromatographic Performance Of Acidic Peptides In RPLC-MS-based Assays 6/30/2022

   We demonstrate how to increase productivity in the lab and mitigate risk through increased reproducibility, recovery, and robustness of assays performed in the development and manufacturing of biopharmaceutical drug products.

9. Achieving Equivalent Results When Transferring Methods While Using Different Vendor’s Equipment 6/29/2022

   We demonstrate method transfer and migration between instruments that successfully replicates methods, producing equivalent chromatographic separation and analytical results generated by the method to the results obtained on the comparable LC systems.

10. A Look At Column Reproducibility: Analysis Of Endogenous Caffeine And Metabolites In Human Urine 6/28/2022

    In this study demonstrates how the HSS T3 stationary phase is a reliable stationary phase, as three batches made over a five-year span provide comparable results.