Bioprocessing Application Notes & Case Studies

  1. Chromabolt® Prepacked Chromatography Columns Performance Guide

    Prepacked chromatography columns provide an economic advantage by freeing up end users valuable time and resources by eliminating manual packing and cleaning.

  2. Improving Documentation Change Control Process

    Changing existing or creating new documentation typically involves multiple disciplines and can have long lead times. CRB was able to help a biotech company improve their Global Documentation Change Control Process quickly and easily.

  3. Thermoinducible Expression Of DNA Polymerase I

    Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) is a unique, robust, and purpose-built solution for microbial fermentation applications, offering flexibility, ease-of-use, and efficiency found in single-use systems. This comparison of the HyPerforma S.U.F.s versus stainless steel fermentors demonstrates that the product yield and catalytic activity of the expressed enzyme were not significantly different.

  4. Production Of Proteinase K: Single-Use vs. Stainless Steel For Microbial Fermentation

    The Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) is a unique and rigorous solution for microbial fermentation applications, offering the flexibility, ease of use, and efficiency found in single-use systems. This comparison of the HyPerforma S.U.F.s versus stainless steel fermentors demonstrates equivalent results, with the additional benefit of no fouling of the exhaust filter.

  5. Production Of Antibody Fragments By S. cerevisiae In The HyPerforma Single-Use Fermentor

    Advances in single-use bioprocess technology have led to the development of the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.), the first specifically designed fermentor of its kind.

  6. Single-Step Purification Of Raw Starch Digesting α-Amylase

    In the case of enzymes, each additional purification step can lead to a proportional loss of enzymatic activity. This makes a single-step purification strategy for enzymes highly appealing.

  7. Development Of Non-Affinity Based Purification Platform For Neutral/Basic IgMs

    The biological properties of IgM antibodies make them very effective vehicles for in vitro diagnostics and therapeutics. However, purification of IgM antibodies is far more difficult than that for the more common IgG antibodies due to their complex structural and biochemical characteristics. Furthermore, the commonly available affinity chromatography supports (Protein A and Protein G) have little or no binding capacity for IgM.

  8. Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison Of Various Mixed-Mode Media To Determine The Optimal Choice For Efficient Purification Process

    In the purification of monoclonal antibodies, the separation of monomers from product-related impurities is a major challenge. Here, we highlight the importance of screening various media to determine the optimal one for an efficient purification process and illustrate that CHT Ceramic Hydroxyapatite is an effective mixed-mode chromatography media.

  9. Zydis® ODT - Improved Therapeutic Profile

    Zydis® fast dissolve formulation for Zelapar® (Selegiline anti-Parkinson’s compound) had a positive impact on the patient compliance as compared to the Selegiline traditional tablets.

  10. Revolutionizing Allergy Therapy

    ALK-Abelló is a global research-driven pharmaceutical company that focuses on the prevention, diagnosis and treatment of allergies. Before ALK-Abelló partnered with Catalent, all marketed allergen immunotherapies were delivered by injection, resulting in a limited patient population and reduced patient adherence. Catalent worked with the client to apply Zydis Fast Dissolve Technology to their therapy. The overall result was a marked improvement in patient tolerability, preference and compliance.