Bioprocessing Application Notes & Case Studies

1. Complete Single-Use ADC Technology From Development Through Scale-up
   11/19/2018

   MilliporeSigma was commissioned to perform the technology transfer and optimization of bioprocess chemistry and associated analytical methods for the production of antibody-drug conjugate ADC-X.

2. Multidimensional Scale-Up Of A Monoclonal Antibody Capture Step Contributes To Shortened Time To Market
   10/10/2018

   Fast and efficient process development and scale-up contributes to a shortened time to market. This application note demonstrates a multidimensional scale-up (change of both column diameter and bed height) of a mAb capture step, using the ÄKTA pilot 600 chromatography system.

3. Process-Economy Simulation Of mAb Capture Step With Protein A Chromatography Resin
   10/10/2018

   Calculation examples that highlight scenarios where the enhanced capacity and alkaline‑stability of MabSelect PrismA can provide process economic benefits to large‑scale mAb processing.

4. Removal Of DNA Contamination During Process Purification
   10/3/2018

   Cell culture production of recombinant proteins results in protein sample contaminated with host cell DNA. This can lead to increased viscosity of the feedstream and interfere with downstream purification steps. Design a process purification process with these new strategies.

5. Use Of Sodium Hydroxide For Cleaning And Sanitization Of Chromatography Resins And Systems
   10/2/2018

   This application note examines these aspects of using sodium hydroxide as a cleaning and sanitizing agent.

6. Lifetime Performance Study Of MabSelect™ PrismA During Repeated Cleaning‑In‑Place Cycles
   10/2/2018

   A demonstration of the long‑term chromatographic performance of MabSelect PrismA during repeated purification cycles using 0.5 M NaOH for CIP.

7. Capacity And Performance Of MabSelect™ PrismA Protein A Chromatography Resin
   10/2/2018

   This application note demonstrates the binding capacity of MabSelect PrismA in comparison with its predecessor products for both polyclonal and monoclonal antibodies.

8. Single-Step Influenza And Dengue Virus Purification With Mixed-Mode CHT Ceramic Hydroxyapatite XT Media
   9/21/2018

   As a media for simple, fast, scalable, and reproducible viral purification, CHT XT Media meets the demand for a media with excellent lifetime and single-step purification functionality for process economics.

9. S.U.B. Enhancements For High-Density Perfusion Cultures
   9/20/2018

   This study presents how strategic enhancements to the sparge and agitation systems of Thermo Scientific HyPerforma S.U.B.s have revealed the potential for a three- to four-fold improvement in mixing and mass transfer performance compared to legacy S.U.B. designs.

10. Mass Transfer Of Single-Use Fermenters To Stirred Glass Bioreactors
    9/20/2018

    The aggressive performance demands of industrial microbiology have limited the conversion of traditional fermentation processes into single-use systems. In order to address the unique needs of microbial applications, single-use fermentors have been designed to meet the requirements of microbial fermentation instead of being modified from a cell culture bioreactor.