Stem cell-derived cortical neurospheres are of great interest in basic neurobiology research and drug research. Cultivation in controlled, stirred-tank bioreactors can improve the homogeneity of neural spheroid cultures and allows culture scale-up.The ability to generate high numbers of homogenous human iPSC-derived neurospheres will contribute towards personalized drug testing in microphysiological systems. This application note demonstrates using a DASbox Mini Bioreactor System for the generation of human induced pluripotent stem cell (hiPSC) spheroids and subsequent neural differentiation.
A helpful guide through all steps of a bioprocess, starting from the preparation of the inoculum to the preparation and operation of the vessels and bioprocess systems, the bioprocess run itself, and the analysis of samples.
A major challenge in downstream purification of mAbs is the formation and removal of aggregates. CHT XT is the newest addition to the CHT family. This study shows that CHT XT is capable of minimizing the aggregate content during mAb purification. It can also reduce other product- and process-related impurities to significantly low levels. These data demonstrate that CHT XT is an exceptional tool for polish purification of monoclonal antibodies.
Compare two purification strategies and their effectiveness to offer high product yields and clearance of acidic impurities from the expression host cells.
Protein A affinity purification has multiple limitations in terms of productivity at manufacturing scale and the costs associated with making commercial antibodies, especially biosimilars. There could be a viable alternative, an ion exchange (IEX) resin, Nuvia S, for the capture purification of such biomolecules. Read instances where its use has resulted in a productive and economical purification workflow.
Biosimilar purification costs constitute more than half of the total development costs. A significant number of these costs are due to the expense of Protein A–based media. This application note proposes an alternate but equally effective approach to biosimilar purification, by substituting a less expensive ion exchange resin for the affinity capture step.
The large size of IgMs makes their purification very challenging. See how Bio-Rad's new strong anion exchange resin (AEX), Nuvia HP-Q, has the ability to overcome the multiple issues faced when purifying large biomolecules.
In this study, we successfully demonstrated large-scale culture of human adipose-derived mesenchymal stem cells (AdMSCs) in an industrial, single-use vessel at 3.75 L scale and demonstrate the fact that the process can be conveniently scaled up to industrial levels of production without having to rely on stainless steel culture facilities.
Alternative to water baths for thawing frozen cells
Freezing cells without liquid nitrogen
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