Clarisolve® depth filter with pDADMAC flocculant pretreatment demonstrates a significant improvement in filtration efficiency, including a reduction in supernatant turbidity and improved volumetric throughput compared to conventional filters. The purification process removes the residual polymer to a concentration less than 1 ppm, where it showed no in vitro cytotoxicity and hemolytic concerns, and could be used as a reasonable target for acceptable polymer clearance. Overall, pDADMAC treatment in combination with Clarisolve® depth filters provides an effective solution for harvesting high-cell-density cultures and can be easily incorporated into current clarification platforms.
HTST pasteurization is a highly effective method to inactivate viruses. It involves the rapid heating of the material to a predetermined temperature (>100 °C) and holding it at that temperature for a specified time to inactivate adventitious viral agents that may be present. This technology has been typically used as an in-house treatment for high risk bioreactor feeds, such as glucose, and is becoming an integral part of many biopharmaceutical companies’ viral risk-mitigation strategies.
The benchtop Cellicon™ Perfusion Solution is designed to meet your perfused seed train challenges. It consists of a controller and a flat sheet cell retention filter with a single-use assembly running in tangential flow filtration mode. This easy‑to‑use solution increases perfusion process efficiency, and provides real-time monitoring and control for reliable and consistent performance.
By amplifying multiple targets within a conserved region of the viral family genome, Blazar detects a much broader range of adventitious viruses as compared to traditional polymerase chain reaction methods.
With the outbreak of COVID-19, the most severe pandemic in the 21st century so far, a feverish search for therapeutic treatment and vaccinations has just started. A substantial number of approaches are focusing on the coronavirus S glycoprotein, a surfaceexposed protein that mediates the entry of the virus into the host cells. The aim of this technical note is to provide a description of the capture and subsequent purification of glycoproteins.
Eshmuno® CMX chromatography resin is a highly selective mixed mode chromatography resin for difficult to purify mAbs, ADCs and fusion proteins. This innovative resin combines weak cation exchange properties with hydrophobic interaction, providing high selectivity for mAb, fusion protein and ADC purification as well as separation of low molecular weight impurities and Host Cell Proteins (HCPs).
Bio4C™ ProcessPad software is a data collection, visualization, and analytics platform that enables bioprocess monitoring, lifecycle management, reporting, investigations, and continued process verification (CPV).
One of the new technologies emerging in the biotech manufacturing market is the use of viral vectors for cell and gene therapy. One commonly used viral vector in this space is lentivirus, due to its robustness to package and deliver target DNA. This work explores a variety of lentivirus harvest clarification options and items for consideration during manufacturing platform development of viral therapy production using suspension cell lines.