Accelerate Development And Optimization Of Cell Culture Media For Upstream Perfusion Processes

Perfusion serves as a method for process intensification, aiming to minimize manufacturing footprint and expenses while boosting both productivity and product quality. However, the development and optimization of cell culture media for perfusion processes can be time-consuming and complex. This process often requires various models and scales, from 1 mL to 3 L. Small-scale models, such as 96-deep well plates and spin tubes, offer high throughput for screening but lack controlled environments and automated perfusion features. Conversely, single-use benchtop bioreactors provide precise control over culture conditions but are not high throughput and necessitate larger volumes of media, making them more suitable for confirmation runs during the later stages of development.

Here, we outline the application of the Mobius® Breez Microbioreactor for the rapid screening and optimization of media formulations tailored for perfusion processes, as well as its role in predicting the performance of larger-scale bioreactors as an alternative to conventional small-scale models. This versatile microbioreactor has several other applications, including expediting and streamlining cell line development and studies of cell metabolism.