product datasheets, catalogs, app notes

1. An Optimized Platform For pDNA Purification, From Lysis To Sterilizing Grade Filtration For Vaccine Production 1/18/2021

   pDNA has become an important therapeutic modality in the form of DNA vaccines and as the basis for RNA vaccines and gene therapy vectors. Purification of pDNA for use as vaccine doses, however, present several challenges. This application note presents a comprehensive set of technologies that are available to optimize the entire workflow.

2. Optimization Strategy And Process Economics Of DNA Digestion In Viral Vector Production For Gene Therapy 12/16/2020

   Benzonase® endonuclease is a high-quality product delivering immense value to viral vector processes. The enzyme activity is strongly influenced by the matrix and optimization of its use should be a mandatory step in process development. In this application note, we highlight an optimization strategy and process economics of DNA digestion in viral vector purification.

3. Tangential Flow Filtration (UF/DF) Of Plasmid DNA 12/16/2020

   Due to their structure, plasmids can sometimes pass through pores that are smaller than their apparent molecular weight. Additionally, the DNA can be shear-sensitive and tends to increase with plasmid size, and the result can be degradation and reduction of the overall yield. Learn how optimization of hydraulic parameters can help you achieve the desired plasmid purity, formulation, and concentration specifications.

4. Cell Harvest, Lysis, Neutralization & Clarification Of Plasmid DNA 12/15/2020

   Plasmid DNA (pDNA) is typically produced via fermentation using a microbial source. Following E. coli fermentation, the primary downstream purification begins with harvesting of the cells, lysis, and clarification. Explore guidance for your pDNA downstream process development, including clarification, TFF, chromatography and sterile filtration unit operations.

5. Intensified Polishing With Single-Pass Tangential Flow Filtration, Anion Exchange Chromatography 11/19/2020

   Single-pass tangential flow filtration (SPTFF) can be used to drive flexibility, lower cost, and higher product quality. Read the application note to learn more.

6. Sterilizing Grade Filtration Unit Operations For Plasmid DNA Processes 11/19/2020

   The large size of pDNA can present a challenge for sterile filtration unit operations within your gene therapy manufacturing since the product can be retained by the filters, leading to both yield loss and low filtration capacity. Optimization of a sterilized grade filtration unit can help increase yield and filtration capacity. Read on to learn more!

7. Chromatographic Purification Of Plasmid DNA 11/19/2020

   Widespread application of pDNA in gene therapy is driving increased demand and as a response, plasmid manufacturing must become more efficient with improved productivity. Intensification of chromatographic steps can help address this demand and has led to an exploration of the use of convective media (monoliths, membranes, fibre based technologies).

8. Bio4C™ Orchestrator Software Data Sheet 11/16/2020

   Bio4C™ Orchestrator connects individual unit operations (UoP) to a centralized platform for unified visibility and monitoring of regulatory compliant biomanufacturing systems and processes.

9. Integritest® 5: Integrity Assurance With Ease And Confidence Datasheet 11/12/2020

   Accurately and reliably verify the integrity of your filters and processing equipment with the portable, easy to implement, and automated Integritest® 5. The Integritest® 5 delivers a simple and intuitive user experience, while providing optional depth of flexibility to fit your process.

10. Bubble Point Testing With Integritest® 5 11/12/2020

    During a bubble point test, pressure decay measurements are taken at increasing applied transmembrane pressures to map the filter’s integrity profile. The Integritest® line of automated integrity testing instruments identifies optimum pressures at which to take measurements based on test parameters and ongoing readings, which reduces the number of measurements needed to reliably map the filters’ profile and return an accurate result.