Featured Articles
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Optimized Protein A Resin To Minimize Elution Buffer Selection Impact
The impact of the elution buffer counter-ion, pH, and molarity were evaluated on the key purification performance parameters of Amsphere A3. In total, five different mAbs were used for the study.
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Enhancing Performance Utilizing A Modular, Lattice Supported Resin Bed
A novel adsorptive device platform having the separation capability of conventional chromatography, with the features and benefits of a cassette format distinguished by a supporting bed scaffold capable of accepting any bead protected by US and International patents.
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Hyper-Productivity In Your mAb Purification Step
Learn how a tenfold increase in productivity of 200 g/L/h compared to the industry norm was possible through an intensified Protein A capture process.
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Key Performance Parameters For High-Capacity Protein A Resin
Currently more than 70 biosimilar mAbs are under development and multiple originator mAbs are going off-patent. Protein A affinity media remains the most important workhorse for the purification of mAbs.
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Protein A Platform For Biosimilar Molecules
Protein A resin remainsan important workhorse for the purification of mAbs. This application note summarizes the key performance parameters for our protein A resin for mAbs and Fc-fusion proteins.
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The Use Of Amsphere A3 Resin To Purify Antibody Fragments
This Q&A outlines the use of Amsphere A3 resin to purify antibody fragments, explains the molecular binding mechanism for VHH single-domain antibodies, and compares dynamic binding capacity data with those of other affinity resins.
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Protein A Resin For Antibody Fragment Purification
This technical poster discusses how an alkali stable protein A resin is used as a platform for fragment mAb purification to overcome downstream challenges associated with non-classical antibody constructs.
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A Lifetime Study For Protein A Resin With CIP Cycle Testing
Resin lifetime is a key parameter that needs to be assessed as this is directly related to both installed resin volume and process costs. The resin should maintain performance parameters over the intended number of process cycles.
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Cell Line Development Platform For Bispecific Antibody Expression
Bispecific antibodies (bsAbs) represent a fast-growing, albeit developmentally challenging, class of molecules offering new therapeutic perspectives. A single-cell system is the most attractive strategy.
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Caustic Stability Of Amsphere A3: Cycling Study With 0.5 M NaOH
Sodium hydroxide is commonly used for cleaning in place of Protein A media and caustic stability is therefore an important asset. During its lifetime, resin performance must remain within set specifications.