INSIGHTS ON UPSTREAM MANUFACTURING

• HCP ELISA And HCP Antibody Coverage Analysis Methods

  Host cell proteins (HCPs) constitute a major group of process-related impurities in biological drugs produced using cell culture technology. Read how HCPs can constitute a significant component of a biopharmaceutical drug developer’s overall risk-management strategy

• Optimized Cell Culture Medium For Scalable Viral Vector Production

  MilliporeSigma’s new cell culture medium offers an improved alternative to classical formulations that can help address the constraints in viral vector manufacturing and scale-up.

• Osmolality Measurement Of Lentiviral Vectors For Cell And Gene Therapy

  In this webinar Lucia Marani, Analytical Development Scientist from Oxford BioMedica about applications of the OsmoTECH osmometer. Kendal Studd, Scientific Application Specialist for Advanced Instruments rounds off the session by presenting a summary of published research showing that in vector manufacturing, osmolality is a factor in control of stability and aggregation for lentivirus and other viral vector types.

• Pellicon® 3 Cassettes Cleaning Methodology For Reuse In Single-Pass Tangential Flow Filtration Mode

  Single-pass tangential flow filtration (SPTFF) can be used to achieve higher final concentrations, optimize product recovery, control volume of intermediate steps, and enable intensified or continuous processing with chromatography or filtration operations. This study demonstrates efficient cleaning strategies to enable multiple reuse of TFF cassettes for high concentration applications with SPTFF.

• Comparison Between Experimental Data And Computational Fluid Dynamics In Mixing Applications

  7/9/2021

  In biomanufacturing processes, mixing is ubiquitous and mixing trials are often necessary to assess if a technical solution is adapted for a given process step or to optimize process parameters. Discover how computational fluid dynamics can help in your mixing trials.

• Raw Materials In Upstream Bioproduction: Challenges And Solutions

  7/6/2021

  Media formulations supporting the production of biologics, such as cell, gene, and protein-based therapies, consist of a large number of raw materials sourced globally from different suppliers. Here’s how to ensure a reliable raw material supply and to mitigate risk from variability. 

• One-Step Isolation And Activation Of Naive And Early Memory T Cells With CTS Dynabeads CD3/CD28

  7/6/2021

  In this application note we demonstrate nearly 100% recovery of naive and early memory T cells using Gibco™ CTS™ Dynabeads™ CD3/CD28 beads. This one-step process of simultaneous T cell isolation and activation yields pure and uniformly activated T cells.

• Highly Sensitive Charge Variant Analysis Of Complex Biopharmaceuticals

  6/29/2021

  This case study explores the use of a capillary electrophoresis device to perform highly selective charge variant separation of complex pharmaceuticals for native intact antibodies while generating excellent quality MS data. This technology allows for in-depth analysis of extremely low abundant charge variants with outstanding method sensitivity.