INSIGHTS ON UPSTREAM MANUFACTURING

• CAR-T Cell Lentiviral Transduction By Spinoculation

  There is a growing need to close and automate manufacturing of chimeric antigen receptor (CAR)-T cell therapies. CAR-T workflows depend on ex vivo gene transfer by lentiviral transduction for therapeutic efficacy. To streamline this step while keeping flexibility during process development, we introduced stand-alone SpinOculation C-Pro protocol software.

• Seed Train Intensification Using High Cell Density Cryopreservation And Specially-Designed Expansion Medium

  Strategies such as process intensification have the potential to create shorter lead times, reduce plant footprint, increase flexibility and reduce COGs. Upstream processes can be intensified using a number of strategies which can deliver significant improvements in speed, flexibility and reliability. In this whitepaper, we explore two of these approaches – High cell density cryopreservation (HCDC) and the use of a perfused seed train including a specially-designed expansion medium.

• HyPerforma 5:1 Single-Use Bioreactor (S.U.B.) Setup And installation Part 3

  This video is part 3 of 3 videos that cover setup and installation topics related to the HyPerforma 5:1 Single-Use Bioreactor, or S.U.B. In this video we will cover probe assembly, making CPC AseptiQuik connections, probe insertion.

• HyPerforma 5:1 Single-Use Bioreactor (S.U.B.) Setup And installation Part 2

  This video is part 2 of 3 videos that cover setup and installation topics related to the HyPerforma 5:1 Single-Use Bioreactor, or S.U.B. In this video we will cover initial BioProcess Container, or BPC, loading, drive shaft insertion, final installation steps.

• HyPerforma 5:1 Single-Use Bioreactor (S.U.B.) Setup And installation Part 1

  This video is part 1 of 3 videos that cover setup and installation topics related to the HyPerforma 5:1 Single-Use Bioreactor, or S.U.B. In this video we will cover 5:1 system component labeling, preparing the load cells.

• Cell Culture Media Filtration: Filter Selection And Sizing

  Upstream bioprocessing is evolving with improvements in cell line development and cell culture media optimization. This application note provides an overview of different filtration options in terms of microbial retention and filtration capacity and provides filter sizing recommendations for MilliporeSigma catalog media and additives. Increased understanding of these different criteria should help biomanufacturers select the right filter for their process needs.

• Production Of Therapeutic Plasmid DNA In Single-Use Fermentors

  The manufacturing of plasmid DNA is becoming a supply bottleneck due to rapidly growing demand for various gene-targeting biotherapeutics. Many producers and end users are moving towards expanding or implementing single-use production facilities to efficiently meet the demand. Here we will review three procedures for production of therapeutic plasmid DNA in single-use fermentors.

• An Integrated Single-Use Platform for Manufacturing Plasmid DNA (pDNA)

  Large quantity, high-quality pDNA vectors are in demand. Discover an integrated single-use platform for manufacturing Plasmid DNA (pDNA) using a high cell density process licensed from Nature Technologies.