INSIGHTS ON UPSTREAM MANUFACTURING

• Overcoming Development And Manufacturing Challenges For Biologic Therapies

  Whether rapidly scaling manufacturing capabilities, shortening supply chains, accelerating the availability of emerging therapies, or reducing the risk of a pharmaceutical shortage, the pharmaceutical manufacturing platform can make all the difference in the success or failure of a biologic therapy.

• Measuring Oxygen Transfer Rate (OTR) Of The Eppendorf Fermentation Vessels

  OTR is a very important parameter of fermentation vessels which affects the selection, design, and scale-up of a bioprocess protocol. Here we describe in detail how we measure the OTR of an Eppendorf fermentation vessel using the industrial standard sulfite depletion method. This short protocol can serve as a reference for the bioprocess professionals who want to perform OTR measurements of Eppendorf’s large collection of fermentation vessels.

• Improving Antibody Production Workflow Using The BioFlo 720 Bioreactor Control System

  To scale upstream bioprocesses, an effective scale-up strategy is required to ensure the reproducibility of both cell culture growth and batch yields at large working volumes, ideally with little or no additional optimization. This study details the use of this new Scale Up Assist feature of the BioFlo 720 Bioreactor Control System to scale CHO batch cultures from 3 L to 10 L BioBLU® Single-Use Vessels and from the 10 L to 50 L Single Use Bioreactor (SUB) bag.

• Real-time Characterization Of Mammalian Cell Culture Bioprocesses By Magnetic Sector MS

  The complexity of mammalian cell culture processes for biopharmaceutical manufacturing has resulted in the rapid development of Process Analytical Technology (PAT) tools aimed at improving batch-to-batch reproducibility through the implementation of real-time process monitoring techniques. In this paper mammalian cell culture processes were characterized upon the analysis of the exhaust-gas composition achieved through the on-line integration of a magnetic sector MS analyser with benchtop bioreactors.

• Cellicon™ Filter And Controller For N-1 Seed Train Intensification

  Perfusion operations deliver high density cell cultures that alleviate the burden of processing large production bioreactor volumes while increasing manufacturing flexibility. Discover Cellicon™ Filter and Controller, specifically designed for N-1 seed train intensification.

• Measuring Cell Density In The HyPerforma S.U.B.s With Aber Futura Neo Single-Use Sensors

  This application note summarizes the sensor’s function, integration into the S.U.B. BPC, and showcases functional cell culture data obtained both internally and with a primary customer.

• Comparison Of N-1 Seed Train Performance In HyPerforma Rocker Bioreactor BPCs Using Renolit 9101 And Aegis 5-14 Films

  This application note shows the performance of CHO-S cells grown in N-1 reactor conditions in both Renolit 9101 and Aegis5-14 films.

• Cultivation Of Sf9 Insect Cells And rSEAP Expression In The HyPerforma Rocker Bioreactor

  This application note describes the cultivation of Sf9 suspension cells in the Thermo Scientific™ HyPerforma™ Rocker Bioreactor with a maximum working volume of 5.0 L.