INSIGHTS ON UPSTREAM MANUFACTURING

• The Importance Of Cell Media Analysis In Upstream Bioprocess Development

  Experts from industry and academia to explore current trends in upstream bioprocessing and the impact of real-time cell media analysis on process improvements and process analytical technologies.

• Unlocking The Potential Of Viral Vectors Through Functional Titer Assays

  This poster reviews two virus-based gene delivery platforms – adenoassociated virus (AAV) and lentivirus (LV) – focusing on the development, validation, and implementation of titration methods used to measure functional titer.

• Production Of Therapeutic Proteins In CHO Cells

  Despite the widespread use of CHO cells in therapeutic protein production, the presence of many CHO cell line options and the need to optimize cell culture conditions for every cell line in a product-specific manner can lead to extended timelines and substantial cost in the development of a cell line for a new biologic.

• New Technology Adoption In Aseptic Filling: Risk Aversion vs. Change Aversion

  Change is an inevitable part of continuous improvement, and risk-mitigated, disruptive advances should be embraced as enthusiastically as incremental change.

• Quantification Of Metabolites In Chemically Defined Media: An Example Of Method Accuracy And Reproducibility

  10/8/2021

  When measuring analytes in complex cell culture media such as CHO and hybridoma media method accuracy and reproducibility are critical. The right analyzer can provide rapid and reliable quantitative cell culture media analysis.

• Bioreactor Monitoring Via ZipChip CE-MS: Quantifying Amino Acids In Spent Growth Media

  10/8/2021

  While many of the media components included to promote and sustain cell growth are small, polar analytes that can be difficult to analyze using conventional techniques we demonstrate a simple and rapid method for quantifying small molecules in spent growth media samples.

• High-Throughput Intact Mass Analysis Of mAb-Based Therapeutics

  10/8/2021

  Intact mass analysis of biotherapeutic proteins using mass spectrometry is a key characterization technique performed from upstream drug development to downstream product analysis. Review a method for high throughput analysis of monoclonal antibodies using a simple approach that can facilitate rapid MS characterization of biotherapeutic proteins.

• Preparing CHO Cells For Higher Productivity By Optimizing A Perfused Seed Train

  10/6/2021

  Existing upstream equipment and/or facility infrastructure can present an impediment to intensification efforts and consequently limit gains in efficiency. This application note describes how N-1 perfusion using an expansion medium supports inoculation of a fed-batch, production bioreactor at a much higher cell density resulting in increased product titers.