INSIGHTS ON UPSTREAM MANUFACTURING

• Expediting Viral-Based Vaccines Development and Increasing Productivity - Part 1 Upstream Processing

  We know that speed is of essence and that scale-up to large-scale production needs to be achieved by strategically balancing risks and speed. This presentation first describes how vaccine developers can accelerate their timelines and develop highly efficient vaccine processes by using high throughput fully automatic multi-parallel bioreactor testing tools, DoE software, and single-use solutions able to operate with mammalian cells, insect cells, and avian cells.

• Integrated Technologies To Accelerate Process Intensification For Viral Vaccine Manufacturing

  With the increasing world-wide demand for viral-based vaccines (VBVs) which includes attenuated and inactivated viral vaccines, as well as viral vector vaccines to protect against the coronavirus known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), faster development times are required to progress VBVs more rapidly into clinical development and then to market. To facilitate process intensification of VBVs including SARS-CoV-2 vaccine candidates, an integrated portfolio of technologies developed by Sartorius is detailed in this article

• Raise Your Upstream IQ: Key Considerations For Optimizing Process Development

  How can you intensify your cell culture processes, in order to keep pace with today’s industry and regulatory expectations?

• Introducing Cellicon™ Perfusion Filter And Controller

  Discover Cellicon™ Perfusion Filter and Controller, a superior perfusion solution for seed train intensification with optimized process control.

• Evaluation Of Novel Cell Retention Technology And Cell Culture Media For N-1 Perfused Seed Train

  Perfusion operations deliver high density cell cultures that alleviate the burden of processing large production bioreactor volumes while increasing manufacturing flexibility. Introducing perfusion to your seed train makes it possible to inoculate a higher quantity of cells for one or more production bioreactors, thus increasing process efficiency. Read our application note to learn more on the use of cell retention technologies to enable perfusion processes.

• Developing TIL Therapies: Sample Collection And Processing

  While it has been advanced over many years, the process of developing TIL therapies still suffers from certain challenges. This article attempts to lay bare some of these challenges and discuss how novel technological solutions can help overcome them.

• Upstream Intensification – Enabling Perfusion Processes With Cell Retention Technologies

  Upstream processes can be intensified using a number of strategies including high cell density cryopreservation, perfused seed train or production processes with high seed fed batch, concentrated fed-batch, ultra-high VCD fed batch, steady-state perfusion, and dynamic perfusion.  In this white paper, we explore perfusion processes and how they are becoming a viable solution.

• Robust Harvest Clarification For Adeno-Associated Viral Vectors Via Depth Filtration

  This article demonstrates that depth filters clarify AAV vectors, helping to overcome the unique separation challenges presented by these important vectors for gene therapy.