Many factors are required to assess and implement a protein A resin into a mAb purification process. Dynamic binding capacity, purity, operating flow rate and caustic compatibility are a few among many evaluation parameters. Resin lifetime is a key parameter that needs to be assessed as this is directly related to both installed resin volume and process costs. The resin should maintain performance parameters over the intended number of process cycles. This technote describes the effects of process cycling and caustic CIP on purification performance of Amsphere A3 over 100 cycles.