Insights On Upstream Manufacturing

  1. Production Of Human Induced Pluripotent Stem Cell-Derived Cortical Neurospheres In The DASbox® Mini Bioreactor System
    1/15/2019

    Stem cell-derived cortical neurospheres are of great interest in basic neurobiology research and drug research. Cultivation in controlled, stirred-tank bioreactors can improve the homogeneity of neural spheroid cultures and allows culture scale-up.The ability to generate high numbers of homogenous human iPSC-derived neurospheres will contribute towards personalized drug testing in microphysiological systems. This application note demonstrates using  a DASbox Mini Bioreactor System for the generation of human induced pluripotent stem cell (hiPSC) spheroids and subsequent neural differentiation.

  2. CHO Cell Cultivation In A DASbox® Mini Bioreactor System And DASGIP® Parallel Bioreactor Systems
    1/15/2019

    A helpful guide through all steps of a bioprocess, starting from the preparation of the inoculum to the preparation and operation of the vessels and bioprocess systems, the bioprocess run itself, and the analysis of samples.

  3. Billion-Cell Hypoxic Expansion Of Human Mesenchymal Stem Cells In BioBLU® 5c Single-Use Vessels
    12/18/2018

    In this study, we successfully demonstrated large-scale culture of human adipose-derived mesenchymal stem cells (AdMSCs) in an industrial, single-use vessel at 3.75 L scale and demonstrate the fact that the process can  be conveniently scaled up to industrial levels of production without having to rely on stainless steel culture facilities.

  4. Water-free vs Water Bath Cell Thawing
    11/29/2018

    Alternative to water baths for thawing frozen cells

  5. Consistent Cord Blood Cryopreservation
    11/29/2018

    Freezing cells without liquid nitrogen

  6. Selecting Filters To Address Your Bioburden Challenges
    11/28/2018

    Sterile filtration of liquids is vital to minimizing microbial contamination and ensuring product safety and integrity. Understanding the criteria for optimum filtering helps streamline product selection.

  7. Industry 4.0 Connects Machines And Data
    10/26/2018

    By applying remote monitoring solutions, advanced modeling, and optimization of manufacturing processes, Industry 4.0 offers improved process reliability and product quality through unlimited possibilities of connecting machines and data.

  8. Amgen’s Digital Transformation: Linking Raw Material Data From Suppliers To Patients
    10/19/2018

    Using its Supplier Relationship Excellence (SRE) program, Amgen opened the lines of communication with suppliers and created a feedback loop where data can be shared to better understand operational performance. 

  9. How Biogen Integrated Process Analytics Technology With End-To-End Supply Chain Transparency
    10/2/2018

    To drive appropriate and dependable critical process control requirements, Biogen explored several novel strategies to increase process and raw material control and optimize communication of data throughout the supply chain.

  10. What Are Exosomes?
    9/28/2018

    Gain an understanding of  exosomes which are recently attracting attentions of researchers as new mediators of cell-cell communication transporting biomolecules such as lipids, proteins, and RNAs in vivo.