Insights On Upstream Manufacturing

  1. Cell Growth Performance In Single-Use Bags

    With the uptake of single-use bags in cell culture processes, some manufacturers have reported inconsistent cell growth. Read how Sartorius has addressed the challenge of cell growth performance in single-use bags with their Flexboy® bags and new Flexsafe® platform.

  2. Adenovirus Production In Single-Use ReadyToProcess WAVE™ 25 Bioreactor System

    Upstream virus production for vaccine manufacturing is typically performed in stirred-tank bioreactor systems, but rocking bioreactor systems offer the benefits of low minimum working volumes that can shorten the seed train prior to inoculation, and agitation can be adjusted to a smooth rocking motion for optimized growth of sensitive cells.  This application note describes the production of adenovirus in HEK293 suspension cells in a rocking bioreactor. Triplicate cultures demonstrate a reproducible and robust batch production of adenovirus.

  3. Optimizing Process Efficiency In Upstream Manufacturing

    Increasing the integration of upstream and downstream processing and moving toward increased automation results in greater optimization of process efficiency - a key goal for biopharmaceutical production.

  4. Upstream Bioprocessing In Food And Feed Applications

    Bioprocessing has been used for decades for the industrial production of beverages and for the production and preservation of food and feed ingredients. The ability to monitor and control critical process parameters is a prerequisite. Read how researchers are tackling the challenges in food and feed applications, both old and new.

  5. Production Of Human iPSC-Derived Cortical Neurospheres In A Mini Bioreactor System

    Stem cell-derived cortical neurospheres are of great interest in basic neurobiology research and drug research. Cultivation in controlled, stirred-tank bioreactors can improve the homogeneity of neural spheroid cultures and allows culture scale-up. 

  6. CHO Cell Cultivation In A DASbox® Mini Bioreactor System And DASGIP® Parallel Bioreactor Systems

    A helpful guide through all steps of a bioprocess, starting from the preparation of the inoculum to the preparation and operation of the vessels and bioprocess systems, the bioprocess run itself, and the analysis of samples.

  7. Billion-Cell Hypoxic Expansion Of Human Mesenchymal Stem Cells In BioBLU® 5c Single-Use Vessels

    In this study, we successfully demonstrated large-scale culture of human adipose-derived mesenchymal stem cells (AdMSCs) in an industrial, single-use vessel at 3.75 L scale and demonstrate the fact that the process can  be conveniently scaled up to industrial levels of production without having to rely on stainless steel culture facilities.

  8. Water-free vs Water Bath Cell Thawing

    Alternative to water baths for thawing frozen cells

  9. Consistent Cord Blood Cryopreservation

    Freezing cells without liquid nitrogen

  10. Selecting Filters To Address Your Bioburden Challenges

    Sterile filtration of liquids is vital to minimizing microbial contamination and ensuring product safety and integrity. Understanding the criteria for optimum filtering helps streamline product selection.