Cell Culture White Papers & Case Studies

  1. Improving DNA Removal from Bioprocess Purification Processes

    Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. By 3M Purification Inc.

  2. Scale-Up Of Microbial Fermentation Using Recombinant E. Coli

    Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures.

  3. Sizing Gold Nanoparticle GNPs For Proper Conjugation

    The inertness and biocompatibility of gold nanoparticles GNPs makes them very useful for specific applications such as medical imaging, drug delivery, gene delivery, scaffold directed assembly and molecular sensing, where the size of GNPs is important. By Beckman Coulter, Inc.

  4. Concepts And Tips For Better Fixed Sample Imaging Results

    Multiple processing steps are required to prepare tissue culture cells for fluorescence microscopy. Experiments are generally classified as being either live or fixed cell microscopy.

  5. An Automated Profiling Application For G9a Histone Methyltransferase And Bromodomain Proteins

    Due to several findings, G9a and BET bromodomain proteins have become the target for numberous drug discovery projects. In this study, we describe a novel, cell-based assay platform that uses the robust, yet simple Enzyme Fragment Complementation (EFC) Technology.

  6. A High-Throughput Static Culture Flask Alternative To Shake Flask Culture

    Irvine Scientific specializes in developing and manufacturing cell culture media for bioproduction. To ensure the utility of the medium, CHO cell lines are tested for density, viability and productivity. By Brandy Nunez, Brian Canna, MBA, and Jessie Ni, PhD

  7. Analysis Of Dialyzed Flask Technology For Antibody Production

    Efficient and cost effective hybridoma culture is essential to small and large scale monoclonal antibody production for research purposes. By Denis Phichith, PhD and Brian Canna, MBA

  8. Validation Study Of The Vi-CELL™ XR For Automated Cell Counting Of Dendritic Cells

    Dendritic cells (DC) population is a key functional constituent of cell based immunotherapy drugs. The correct cell count and adequate viability of DC are one of the quality control criteria for the final product release. Number of viable DC is historically determined by microscopy using manual counting method – Bürker chamber and trypan blue dye for dead cell exclusion. By Iveta Bottova, PhD

  9. Efficient, High-Titer Monoclonal Antibody Production In A Fed-Batch Process

    This application note shows the feasibility of monoclonal antibody (MAb) production in fed-batch processes using the single-use Xcellerex™ XDR-200 stirred-tank and ReadyToProcess WAVE™ 25 rocking bioreactor systems.

  10. Bio-Monolith Protein A Monitors Monoclonal Antibody Titer From Cell Cultures

    In the biopharmaceutical industry, downstream processing for monoclonal antibody production typically includes three chromatographic steps; capture, intermediate purification, and polishing. By Phu T Duong, Agilent Technologies, Inc.