Cell Culture White Papers & Case Studies

  1. Fast Magnetic Bead Purification Of Interacting Cellular Proteins 12/14/2012

    Biomedical research, and in particular cancer research, is progressing from focusing on small numbers of molecules or cellular events to global functional analysis.

  2. Why Magnetic Beads Have Several Advantages Over Direct Coupling 12/14/2012

    The use of magnetic beads as the solid phase in ELISA (Enzyme-linked immunosorbent assay) has several advantages over direct coupling to the wells. These advantages include an increased available surface area and an even distribution of beads throughout the sample providing rapid and sensitive detection of low analyte concentrations (1. Liabakk et al).

  3. Automated Tissue Culture Cell Fixation And Staining In Microplates - Combination Washer Dispenser To Prepare Samples For Cellular Imaging 8/24/2012

    Cellular imaging is becoming more important as a research tool in drug discovery and biomedical research. Low experimental sample numbers has historically allowed for manual implementation of the fixation and staining processes required prior to image analysis. By Paul Held PhD and Bridget Bishop, BioTek Instruments, Inc.

  4. Protein A Alternatives: Mixed Mode Resins For High Throughput mAb Feedstocks 8/16/2012

    Mixed mode resins can serve as an alternative to protein A in intermediate processing. Implementation of mixed mode resins can reduce water required for filtration and time  for column loading.
     

  5. Chromatography Column Protection With 3M Purification LifeASSURE™ PLA Series Membrane Filters 9/14/2011
    Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.
  6. Improving DNA Removal from Bioprocess Purification Processes

    Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. By 3M Purification Inc.

  7. Scale-Up Of Microbial Fermentation Using Recombinant E. Coli

    Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures.

  8. Monitor The Effect Of Histone Methylase And Demethylase Inhibitors That Induce Apoptosis In Cancer Cell Lines

    Histone lysine methylation is a reversible process, dynamically regulated by both lysine methyltransferases and demethylases. In general, methylation of histone H3 lysine 4 (H3K4me), H3K36, or H3K79 is associated with active transcription, whereas methylation of H3K9, H3K27, or H4K20 is associated with gene silencing.

  9. Streamlining Cell Culture Liquid Handling

    Successful manipulation of cell cultures relies on the ability to maintain sterility and to avoid cross-contamination between cell lines.

  10. Sizing Gold Nanoparticle GNPs For Proper Conjugation

    The inertness and biocompatibility of gold nanoparticles GNPs makes them very useful for specific applications such as medical imaging, drug delivery, gene delivery, scaffold directed assembly and molecular sensing, where the size of GNPs is important. By Beckman Coulter, Inc.