Bio-Monolith Protein A Monitors Monoclonal Antibody Titer From Cell Cultures

By Phu T Duong, Agilent Technologies, Inc.

In the biopharmaceutical industry, downstream processing for monoclonal antibody production typically includes three chromatographic steps; capture, intermediate purification, and polishing. Protein A is frequently used as a capturing step, which results in excellent throughput (that is, capacity and speed) while playing an important role in concentrating the target molecule, immunoglobulin. To monitor monoclonal antibody titer and yield from cell-culture supernatants before expensive preparative and large amounts of protein A are employed, a small (analytical) scale procedure is necessary to determine the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody product. In this application note, prepacked Agilent Bio-Monolith Protein A columns were used to illustrate the quick capture of monoclonal antibody titer from cell supernatant.