Cell Culture White Papers & Case Studies

  1. Can We Win The War Against Mycoplasma Contamination? 11/12/2015

    Mycoplasma maybe the smallest class of free living microorganism, but the consequences of a contamination in a cell batch during bioprocessing can be huge.

  2. Better Characterization of Biomolecules Using Agilent AdvanceBio Reversed-Phase Columns 7/2/2013

    Reversed-phase (RP) is one of the three key techniques used in biochromatography and is particularly valuable because of its compatibility with LC/MS detection. And small particle improvements, such as those found in Agilent ZORBAX RRHD 300A, 1.8 um columns, make RP an attractive choice for many biopharmaceutical applications.

  3. Efficiency Test Of ReadyToProcess™ Columns 5/1/2013

    ReadyToProcess™ columns are prepacked, prequalified, and presanitized process chromatography columns suited for purification of biopharmaceuticals (e.g., proteins, vaccines, plasmids, and viruses) for clinical phase I and II studies. ReadyToProcess columns are available with several media at different volumes (1, 2.5, 10, and 20 L).

  4. Automate BRD4 Bromodomain TR-FRET Assays For HTS Applications 2/12/2013

    Post-translational histone modifications are critical for modulation of chromatin structure by direct DNA interaction and indirect molecular interactions with recruited nuclear proteins. By Pete Brescia, Brad Larson, and Peter Banks, BioTek Instruments, Inc., and D. Bochar and L. Blazer, Cayman Chemical Co.

  5. Automating A Direct, Cell-Based, Target-Compound Interaction Assay For G9a And BET Bromodomain Proteins 2/11/2013

    Current biochemical assay technologies examine enzyme activity and G9a and BET bromodomain protein inhibition. Cell-based assays for these epigenetic players were previously limited to the detection of specific histone modifications using antibodies, with antibody specificity being an issue. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  6. Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells 2/10/2013

    Pharmaceutical companies are increasingly exploring new biologic and biosimilar products, and thus increasing monoclonal antibody (mAb) immunotherapeutic research. By Tracy Worzella, Promega Corporation and Brad Larson, Applications Department, BioTek Instruments, Inc.

  7. Fast Magnetic Bead Purification Of Interacting Cellular Proteins 12/14/2012

    Biomedical research, and in particular cancer research, is progressing from focusing on small numbers of molecules or cellular events to global functional analysis.

  8. Why Magnetic Beads Have Several Advantages Over Direct Coupling 12/14/2012

    The use of magnetic beads as the solid phase in ELISA (Enzyme-linked immunosorbent assay) has several advantages over direct coupling to the wells. These advantages include an increased available surface area and an even distribution of beads throughout the sample providing rapid and sensitive detection of low analyte concentrations (1. Liabakk et al).

  9. Protein A Alternatives: Mixed Mode Resins For High Throughput mAb Feedstocks 8/16/2012

    Mixed mode resins can serve as an alternative to protein A in intermediate processing. Implementation of mixed mode resins can reduce water required for filtration and time  for column loading.
     

  10. Chromatography Column Protection With 3M Purification LifeASSURE™ PLA Series Membrane Filters 9/14/2011
    Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.