Application Note

Scale-Up Of Microbial Fermentation Using Recombinant E. Coli

Source: Thermo Fisher Scientific

By Jason Brown, Paula Decaria, Nephi Jones, and Tommy Smith

Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures. As expression systems are moved to production volumes, they can easily be scaled from a benchtop fermentor (or flask) to the S.U.F. Typically, with other single-use bioreactors used as fermentors, culture procedures have to be modified substantially and risk negatively affecting the organism or plasmid because of the performance constraints of those bioreactors. The HyPerforma S.U.F. was designed to meet the unique requirements of microbial fermentation instead of being modified from a cell culture bioreactor.