Cell Culture White Papers & Case Studies

  1. Concepts And Tips For Better Fixed Sample Imaging Results

    Multiple processing steps are required to prepare tissue culture cells for fluorescence microscopy. Experiments are generally classified as being either live or fixed cell microscopy.

  2. An Automated Profiling Application For G9a Histone Methyltransferase And Bromodomain Proteins

    Due to several findings, G9a and BET bromodomain proteins have become the target for numberous drug discovery projects. In this study, we describe a novel, cell-based assay platform that uses the robust, yet simple Enzyme Fragment Complementation (EFC) Technology.

  3. An In Vitro Microplate Assay That Can Monitor IL-6 Secretions From SKOV-3 Ovarian Carcinoma Cells

    Epidermal growth factor receptor (EGFR) is expressed in up to 70% of epithelial ovarian cancers (EOCs). By Brad Larson and Peter Banks, BioTek Instruments, Inc., Nicolas Pierre, CisBio US Inc., and Stephanie Georgiou, Enzo Life Sciences

  4. A High-Throughput Static Culture Flask Alternative To Shake Flask Culture

    Irvine Scientific specializes in developing and manufacturing cell culture media for bioproduction. To ensure the utility of the medium, CHO cell lines are tested for density, viability and productivity. By Brandy Nunez, Brian Canna, MBA, and Jessie Ni, PhD

  5. Analysis Of Dialyzed Flask Technology For Antibody Production

    Efficient and cost effective hybridoma culture is essential to small and large scale monoclonal antibody production for research purposes. By Denis Phichith, PhD and Brian Canna, MBA

  6. Validation Study Of The Vi-CELL™ XR For Automated Cell Counting Of Dendritic Cells

    Dendritic cells (DC) population is a key functional constituent of cell based immunotherapy drugs. The correct cell count and adequate viability of DC are one of the quality control criteria for the final product release. Number of viable DC is historically determined by microscopy using manual counting method – Bürker chamber and trypan blue dye for dead cell exclusion. By Iveta Bottova, PhD

  7. Efficient, High-Titer Monoclonal Antibody Production In A Fed-Batch Process

    This application note shows the feasibility of monoclonal antibody (MAb) production in fed-batch processes using the single-use Xcellerex™ XDR-200 stirred-tank and ReadyToProcess WAVE™ 25 rocking bioreactor systems.

  8. Bio-Monolith Protein A Monitors Monoclonal Antibody Titer From Cell Cultures

    In the biopharmaceutical industry, downstream processing for monoclonal antibody production typically includes three chromatographic steps; capture, intermediate purification, and polishing. By Phu T Duong, Agilent Technologies, Inc.

  9. Z-Stacking Of Single Plane Digital Widefield Fluorescent Images

    Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  10. Use Of Phase Contrast Imaging To Track Morphological Cellular Changes Due To Apoptotic Activity

    Phenotypic screening, or the determination of the effects (phenotypes) that a molecule has on a cell, tissue, or whole organism, dates back to earliest drug discovery efforts. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.