Upstream Processing White Papers and Case Studies
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Imaging And Analysis Of A High Density Cell Migration Assay
Complex biological or environmental signaling events can lead to cell migration, the movement of cells from one location to another. The migration of cells commonly occurs during both normal biological processes such as the development of an organism or marking pathological events such as cancer metastasis. Automated processes in higher density formats improve throughput greatly increasing the usefulness of performing migration assays at a larger scale. This study evaluated the use of a high density cell exclusion assay in 384- well format for investigation of cell migration using a cell line stably expressing green fluorescent protein (GFP).
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Kinetic Ligand Binding And 3D Tumor Invasion Technologies To Assess Drug Residence Time And Anti-metastatic Effects
Metastasis, the spread of cancer cells from the original tumor to secondary locations within the body, is linked to approximately 90% of cancer deaths. The expression of chemokine receptors, such as CXCR4 and CCR7, is tightly correlated with the metastatic properties of breast cancer cells. In vivo, neutralizing the interaction of CXCR4 and its known ligand, SDF1-α (CXCL12), significantly impaired the metastasis of breast cancer cells and cell migration.
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Sizing Gold Nanoparticle GNPs For Proper Conjugation
The inertness and biocompatibility of gold nanoparticles GNPs makes them very useful for specific applications such as medical imaging, drug delivery, gene delivery, scaffold directed assembly and molecular sensing, where the size of GNPs is important. By Beckman Coulter, Inc.
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An Automated Profiling Application For G9a Histone Methyltransferase And Bromodomain Proteins
Due to several findings, G9a and BET bromodomain proteins have become the target for numberous drug discovery projects. In this study, we describe a novel, cell-based assay platform that uses the robust, yet simple Enzyme Fragment Complementation (EFC) Technology.
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An In Vitro Microplate Assay That Can Monitor IL-6 Secretions From SKOV-3 Ovarian Carcinoma Cells
Epidermal growth factor receptor (EGFR) is expressed in up to 70% of epithelial ovarian cancers (EOCs). By Brad Larson and Peter Banks, BioTek Instruments, Inc., Nicolas Pierre, CisBio US Inc., and Stephanie Georgiou, Enzo Life Sciences
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Scale-Up Of A Cell-Based Influenza Virus Production Process
The white paper includes a brief discussion around modern vaccine processes, followed by a case study showing the scale-up of upstream and downstream processes for the production of a cell based live attenuated influenza virus using single-use ReadyToProcess technology.
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Automation Of Cell Staining
Automation with the Biomek 4000 platform can improve the throughput and reproducibility of conventional staining of adherent cells.
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The Effect Of Cell Culture Method On Long-Term Primary Hepatocyte Cell Health
Hepatotoxicity studies are an important part of the drug discovery process following lead molecule generation.
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Automated Cell Dispensing And Image-Based Spheroid Formation Tracking
Scientists using cell culture for drug discovery, toxicology, stem cell biology, and basic research realize the critical importance of 3-dimensional (3D) models. Data from cells cultured in a non-physiologic, monolayer format on plastic surfaces has long been suspected to differ from true in vivo physiology, and there is mounting evidence that this difference is slowing the pace of scientific discovery. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc., and Nicky Slawny, 3D Biomatrix, Inc.
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Validation Study Of The Vi-CELLâ„¢ XR For Automated Cell Counting Of Dendritic Cells
Dendritic cells (DC) population is a key functional constituent of cell based immunotherapy drugs. The correct cell count and adequate viability of DC are one of the quality control criteria for the final product release. Number of viable DC is historically determined by microscopy using manual counting method – Bürker chamber and trypan blue dye for dead cell exclusion. By Iveta Bottova, PhD