Upstream Processing White Papers and Case Studies

41. An Automated Profiling Application For G9a Histone Methyltransferase And Bromodomain Proteins

    Due to several findings, G9a and BET bromodomain proteins have become the target for numberous drug discovery projects. In this study, we describe a novel, cell-based assay platform that uses the robust, yet simple Enzyme Fragment Complementation (EFC) Technology.

42. An In Vitro Microplate Assay That Can Monitor IL-6 Secretions From SKOV-3 Ovarian Carcinoma Cells

    Epidermal growth factor receptor (EGFR) is expressed in up to 70% of epithelial ovarian cancers (EOCs). By Brad Larson and Peter Banks, BioTek Instruments, Inc., Nicolas Pierre, CisBio US Inc., and Stephanie Georgiou, Enzo Life Sciences

43. Automation Of Cell Staining

    Automation with the Biomek 4000 platform can improve the throughput and reproducibility of conventional staining of adherent cells. 

44. The Effect Of Cell Culture Method On Long-Term Primary Hepatocyte Cell Health

    Hepatotoxicity studies are an important part of the drug discovery process following lead molecule generation.

45. Automated Cell Dispensing And Image-Based Spheroid Formation Tracking

    Scientists using cell culture for drug discovery, toxicology, stem cell biology, and basic research realize the critical importance of 3-dimensional (3D) models. Data from cells cultured in a non-physiologic, monolayer format on plastic surfaces has long been suspected to differ from true in vivo physiology, and there is mounting evidence that this difference is slowing the pace of scientific discovery. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc., and Nicky Slawny, 3D Biomatrix, Inc.
     

46. Validation Study Of The Vi-CELL™ XR For Automated Cell Counting Of Dendritic Cells

    Dendritic cells (DC) population is a key functional constituent of cell based immunotherapy drugs. The correct cell count and adequate viability of DC are one of the quality control criteria for the final product release. Number of viable DC is historically determined by microscopy using manual counting method – Bürker chamber and trypan blue dye for dead cell exclusion. By Iveta Bottova, PhD

47. Live Cell Hypoxia Determination In A 3D Spheroid Model

    Cell-based assays provide a wealth of drug screening information compared to their biochemical counterparts, and at a fraction of the cost and time seen with traditional animal models.

48. Z-Stacking Of Single Plane Digital Widefield Fluorescent Images

    Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

49. Use Of Phase Contrast Imaging To Track Morphological Cellular Changes Due To Apoptotic Activity

    Phenotypic screening, or the determination of the effects (phenotypes) that a molecule has on a cell, tissue, or whole organism, dates back to earliest drug discovery efforts. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

50. Hematoxylin And Eosin Stained Tissue

    Using Color Brightfield Imaging with the Cytation™ 5 to Image Fixed and Stained Tissue. By Paul Held Ph. D., Laboratory Manager, Applications Department, BioTek Instruments, Inc.