Upstream Processing White Papers and Case Studies

  1. Validation Study Of The Vi-CELL™ XR For Automated Cell Counting Of Dendritic Cells

    Dendritic cells (DC) population is a key functional constituent of cell based immunotherapy drugs. The correct cell count and adequate viability of DC are one of the quality control criteria for the final product release. Number of viable DC is historically determined by microscopy using manual counting method – Bürker chamber and trypan blue dye for dead cell exclusion. By Iveta Bottova, PhD

  2. Z-Stacking Of Single Plane Digital Widefield Fluorescent Images

    Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  3. Use Of Phase Contrast Imaging To Track Morphological Cellular Changes Due To Apoptotic Activity

    Phenotypic screening, or the determination of the effects (phenotypes) that a molecule has on a cell, tissue, or whole organism, dates back to earliest drug discovery efforts. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  4. Automated Color Imaging Of Tissues

    Using Color Brightfield Microscopy and Image Stitching to Examine Fixed and Stained Tissues on Microscope Slides. By Paul Held Ph. D., Laboratory Manager, Applications Department, BioTek Instruments, Inc.

  5. Cultivation Of Aggressive Microbial Cultures Of Pichia And Bacillus

    Advances in single-use bioprocess technology have led to the rapid adoption of single-use bioreactors (S.U.B.s) across a broad range of applications within the biotechnology industry.

  6. Comparison Of Assay Methods For The Detection Of Residual Protein A In Biological Therapeutics

    There continues to be a focus on the development of recombinant human monoclonal antibodies (rhuMAb IgGs) for therapeutic use with dozens reaching the market in the last decade.