Upstream Processing White Papers and Case Studies

  1. Improved Systems For pH, DO And Conductivity Measurement In Biopharmaceutical Fermentation 9/7/2011
    When planning a modern 750 liter fermentation plant including cross flow unit, the GEA Diessel company was looking for the best available measurement technology suited for the customer requirements.
  2. Improving DNA Removal from Bioprocess Purification Processes

    Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. By 3M Purification Inc.

  3. Scale-Up Of Microbial Fermentation Using Recombinant E. Coli

    Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures.

  4. Sizing Gold Nanoparticle GNPs For Proper Conjugation

    The inertness and biocompatibility of gold nanoparticles GNPs makes them very useful for specific applications such as medical imaging, drug delivery, gene delivery, scaffold directed assembly and molecular sensing, where the size of GNPs is important. By Beckman Coulter, Inc.

  5. An Automated Profiling Application For G9a Histone Methyltransferase And Bromodomain Proteins

    Due to several findings, G9a and BET bromodomain proteins have become the target for numberous drug discovery projects. In this study, we describe a novel, cell-based assay platform that uses the robust, yet simple Enzyme Fragment Complementation (EFC) Technology.

  6. Validation Study Of The Vi-CELL™ XR For Automated Cell Counting Of Dendritic Cells

    Dendritic cells (DC) population is a key functional constituent of cell based immunotherapy drugs. The correct cell count and adequate viability of DC are one of the quality control criteria for the final product release. Number of viable DC is historically determined by microscopy using manual counting method – Bürker chamber and trypan blue dye for dead cell exclusion. By Iveta Bottova, PhD

  7. Z-Stacking Of Single Plane Digital Widefield Fluorescent Images

    Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  8. Use Of Phase Contrast Imaging To Track Morphological Cellular Changes Due To Apoptotic Activity

    Phenotypic screening, or the determination of the effects (phenotypes) that a molecule has on a cell, tissue, or whole organism, dates back to earliest drug discovery efforts. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  9. Automated Color Imaging Of Tissues

    Using Color Brightfield Microscopy and Image Stitching to Examine Fixed and Stained Tissues on Microscope Slides. By Paul Held Ph. D., Laboratory Manager, Applications Department, BioTek Instruments, Inc.

  10. Cultivation Of Aggressive Microbial Cultures Of Pichia And Bacillus

    Advances in single-use bioprocess technology have led to the rapid adoption of single-use bioreactors (S.U.B.s) across a broad range of applications within the biotechnology industry.