Upstream Processing White Papers and Case Studies

  1. Why Magnetic Beads Have Several Advantages Over Direct Coupling 12/14/2012

    The use of magnetic beads as the solid phase in ELISA (Enzyme-linked immunosorbent assay) has several advantages over direct coupling to the wells. These advantages include an increased available surface area and an even distribution of beads throughout the sample providing rapid and sensitive detection of low analyte concentrations (1. Liabakk et al).

  2. Fast Quantification Of Saliva Samples In Microplates 12/7/2012

    A successful evaluation study of saliva samples in microplates using Tecan’s Infinite F50 microplate reader in combination with Tecan’s Magellan™ data analysis software, using the Saliva Quantification Kit from Greiner Bio-One for the fast detection of saliva samples.

  3. Automated Tissue Culture Cell Fixation And Staining In Microplates - Combination Washer Dispenser To Prepare Samples For Cellular Imaging 8/24/2012

    Cellular imaging is becoming more important as a research tool in drug discovery and biomedical research. Low experimental sample numbers has historically allowed for manual implementation of the fixation and staining processes required prior to image analysis. By Paul Held PhD and Bridget Bishop, BioTek Instruments, Inc.

  4. Protein A Alternatives: Mixed Mode Resins For High Throughput mAb Feedstocks 8/16/2012

    Mixed mode resins can serve as an alternative to protein A in intermediate processing. Implementation of mixed mode resins can reduce water required for filtration and time  for column loading.
     

  5. Micro-Volume Purity Assessment Of Nucleic Acids Using A260/A280 Ratio And Spectral Scanning 6/7/2012

    A common method to determine the purity of biomolecules from sample isolates is by use of a spectrophotometric ratio using absorbance measurements at wavelengths of 260 nm and 280 nm. By Peter Brescia, Applications Scientist, Applications Dept, BioTek Instruments, Inc.

  6. Simplifying And Accelerating The R&D Process With An Electronic Laboratory Notebook 5/24/2012

    e2v Biosensors is a wholly owned subsidiary of e2v technologies that is developing a range of new products for drug discovery and medical diagnostics. One of these products is Visulife, a benchtop analyzer with integrated biochips and reader that enables the rapid and reliable analyses of proteins. 

  7. UV/VIS Absorbance Measurements Application Note 5/18/2012

    Fiber optic spectrometers are ideal in UV/VIS absorbance measurements due to their small size, their ability to be introduced in harsh environments via their fiber interface, and their low cost. UV/VIS absorbance measurements involve a myriad of chemical and biochemical applications with several potential research and industrial end uses. This application note discussed UV/VIS absorbance measurements, common system configurations used in these measurements, the advantages of fiber optics, instrument control software, and more.

  8. Solar Measurements Application Note 5/18/2012

    Avantes has designed and developed spectroscopy and spectroradiometry systems for the solar industry. These systems can be used in solar applications like process control in the manufacture of thin film photovoltaic panels, direct solar measurements, solar simulator characterization, and more. This application note discusses how Avantes’ spectroscopy and spectroradiometry systems are used in solar measurement applications.

     

  9. Fluorescence Spectroscopy: A More Energy Efficient Light Source For Biomedial Research 5/18/2012

    The measurement needs of fluorescence vary greatly depending upon the application. Avantes has worked closely with a number of researchers and process engineers to facilitate fluorescence measurements in research, clinical and industrial environments.

  10. IR-Based Protein Quantitation Surpasses Colorimetric Assay Quantitation And Results Are Independent Of Detergents, Reducing Agents And Analysis Time 3/26/2012
    Accurate protein quantitation in complex mixtures is crucial for obtaining reproducible biological and biochemical data for both upstream and downstream applications.