Upstream Processing White Papers and Case Studies
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Challenges And Solutions Of Scalable Cultivation For Anchorage Dependent Cells In Single-Use Bioreactor
Microcarrier (MC)-based suspension culture processes have been used to culture anchorage-dependent cells in bioreactors. However, scale-up of the process for high cell growth and productivity poses many challenges. By Brian Lee, Yas Hashimura, Daniel Giroux, Marcos Sousa, Joao Clemente, Manuel Carrondo, Siddharth Gupta, Michael McCaman, and Yong-Jae Kim
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Live Cell Hypoxia Determination In A 3D Spheroid Model
Cell-based assays provide a wealth of drug screening information compared to their biochemical counterparts, and at a fraction of the cost and time seen with traditional animal models.
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Z-Stacking Of Single Plane Digital Widefield Fluorescent Images
Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.
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Use Of Phase Contrast Imaging To Track Morphological Cellular Changes Due To Apoptotic Activity
Phenotypic screening, or the determination of the effects (phenotypes) that a molecule has on a cell, tissue, or whole organism, dates back to earliest drug discovery efforts. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.
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Hematoxylin And Eosin Stained Tissue
Using Color Brightfield Imaging with the Cytation™ 5 to Image Fixed and Stained Tissue. By Paul Held Ph. D., Laboratory Manager, Applications Department, BioTek Instruments, Inc.
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Automated Color Imaging Of Tissues
Using Color Brightfield Microscopy and Image Stitching to Examine Fixed and Stained Tissues on Microscope Slides. By Paul Held Ph. D., Laboratory Manager, Applications Department, BioTek Instruments, Inc.
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Practices Using Carbon In The Production Of Fine Chemicals
Carbon has been used in the pharmaceutical industry for many years to reduce impurities in production processes. These impurities are typically derived from chemical reactions producing colored by-products. Carbon is used to reduce chemical impurities as it has many structural characteristics that are suitable.
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Cultivation Of Aggressive Microbial Cultures Of Pichia And Bacillus
Advances in single-use bioprocess technology have led to the rapid adoption of single-use bioreactors (S.U.B.s) across a broad range of applications within the biotechnology industry.
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Comparison Of Assay Methods For The Detection Of Residual Protein A In Biological Therapeutics
There continues to be a focus on the development of recombinant human monoclonal antibodies (rhuMAb IgGs) for therapeutic use with dozens reaching the market in the last decade.