Upstream Processing White Papers and Case Studies
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Evaluating Real Time Viability Detectors
5/9/2012
Rapid microbiological methods have been around for years but have yet to make a significant impact on pharma manufacturing despite the advantages they offer in cost savings and process improvement.
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IR-Based Protein Quantitation Surpasses Colorimetric Assay Quantitation And Results Are Independent Of Detergents, Reducing Agents And Analysis Time
3/26/2012
Accurate protein quantitation in complex mixtures is crucial for obtaining reproducible biological and biochemical data for both upstream and downstream applications.
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Characterization Tool For Poly(2-Oxazoline)s, ESI-Q-TOF MS/MS (Electrospray Ionization Quadruple Time-Of-Flight Mass Spectrometry)
11/13/2011
Electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) has been used for the detailed characterization of poly(2-oxazoline)s.
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The maXis Impact™ Mass Spectrometer Identifies >1600 Proteins In A Single LC-MS/MS Experiment
11/13/2011
The analysis of complex proteomic samples is one of the most demanding tasks in proteomics studies. The sample complexity requires the utmost speed, dynamic range and sensitivity from an analytical system to be used effectively.
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Perfusion And Single-Use Bioreactors: A Partnership For Scale Up
11/3/2011
Perfusion has emerged as a viable technology for the commercial production of biopharmaceuticals. Perfusion solutions can offer very cost-efficient scale up solutions, driving down operating costs due to the smaller equipment and facilities needed as compared to batch or fed-batch systems.
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Chromatography Column Protection With 3M Purification LifeASSUREā¢ PLA Series Membrane Filters
9/14/2011
Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.
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Improved Systems For pH, DO And Conductivity Measurement In Biopharmaceutical Fermentation
9/7/2011
When planning a modern 750 liter fermentation plant including cross flow unit, the GEA Diessel company was looking for the best available measurement technology suited for the customer requirements.
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Improving DNA Removal from Bioprocess Purification Processes
Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. By 3M Purification Inc.
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Scale-Up Of Microbial Fermentation Using Recombinant E. Coli
Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures.
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Monitor The Effect Of Histone Methylase And Demethylase Inhibitors That Induce Apoptosis In Cancer Cell Lines
Histone lysine methylation is a reversible process, dynamically regulated by both lysine methyltransferases and demethylases. In general, methylation of histone H3 lysine 4 (H3K4me), H3K36, or H3K79 is associated with active transcription, whereas methylation of H3K9, H3K27, or H4K20 is associated with gene silencing.