Upstream Processing White Papers and Case Studies

31. Characterization Tool For Poly(2-Oxazoline)s, ESI-Q-TOF MS/MS (Electrospray Ionization Quadruple Time-Of-Flight Mass Spectrometry) 11/13/2011
    Electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) has been used for the detailed characterization of poly(2-oxazoline)s.
32. The maXis Impact™ Mass Spectrometer Identifies >1600 Proteins In A Single LC-MS/MS Experiment 11/13/2011
    The analysis of complex proteomic samples is one of the most demanding tasks in proteomics studies. The sample complexity requires the utmost speed, dynamic range and sensitivity from an analytical system to be used effectively.
33. Chromatography Column Protection With 3M Purification LifeASSURE™ PLA Series Membrane Filters 9/14/2011
    Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.
34. Improved Systems For pH, DO And Conductivity Measurement In Biopharmaceutical Fermentation 9/7/2011
    When planning a modern 750 liter fermentation plant including cross flow unit, the GEA Diessel company was looking for the best available measurement technology suited for the customer requirements.
35. Improving DNA Removal from Bioprocess Purification Processes

    Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. By 3M Purification Inc.

36. Scale-Up Of Microbial Fermentation Using Recombinant E. Coli

    Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures.

37. Monitor The Effect Of Histone Methylase And Demethylase Inhibitors That Induce Apoptosis In Cancer Cell Lines

    Histone lysine methylation is a reversible process, dynamically regulated by both lysine methyltransferases and demethylases. In general, methylation of histone H3 lysine 4 (H3K4me), H3K36, or H3K79 is associated with active transcription, whereas methylation of H3K9, H3K27, or H4K20 is associated with gene silencing.

38. Imaging And Analysis Of A High Density Cell Migration Assay

    Complex biological or environmental signaling events can lead to cell migration, the movement of cells from one location to another. The migration of cells commonly occurs during both normal biological processes such as the development of an organism or marking pathological events such as cancer metastasis. Automated processes in higher density formats improve throughput greatly increasing the usefulness of performing migration assays at a larger scale. This study evaluated the use of a high density cell exclusion assay in 384- well format for investigation of cell migration using a cell line stably expressing green fluorescent protein (GFP).

39. Kinetic Ligand Binding And 3D Tumor Invasion Technologies To Assess Drug Residence Time And Anti-metastatic Effects

    Metastasis, the spread of cancer cells from the original tumor to secondary locations within the body, is linked to approximately 90% of cancer deaths. The expression of chemokine receptors, such as CXCR4 and CCR7, is tightly correlated with the metastatic properties of breast cancer cells. In vivo, neutralizing the interaction of CXCR4 and its known ligand, SDF1-α (CXCL12), significantly impaired the metastasis of breast cancer cells and cell migration.

40. Sizing Gold Nanoparticle GNPs For Proper Conjugation

    The inertness and biocompatibility of gold nanoparticles GNPs makes them very useful for specific applications such as medical imaging, drug delivery, gene delivery, scaffold directed assembly and molecular sensing, where the size of GNPs is important. By Beckman Coulter, Inc.