Upstream Processing White Papers and Case Studies

  1. Automating A Direct, Cell-Based, Target-Compound Interaction Assay For G9a And BET Bromodomain Proteins 2/11/2013

    Current biochemical assay technologies examine enzyme activity and G9a and BET bromodomain protein inhibition. Cell-based assays for these epigenetic players were previously limited to the detection of specific histone modifications using antibodies, with antibody specificity being an issue. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  2. Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells 2/10/2013

    Pharmaceutical companies are increasingly exploring new biologic and biosimilar products, and thus increasing monoclonal antibody (mAb) immunotherapeutic research. By Tracy Worzella, Promega Corporation and Brad Larson, Applications Department, BioTek Instruments, Inc.

  3. AlphaLISA Assays An Alternative To Conventional ELISAs 12/14/2012

    AlphaLISA is a homogeneous, no-wash alternative to conventional ELISAs based on PerkinElmer’s bead-based Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology.

  4. Fast Magnetic Bead Purification Of Interacting Cellular Proteins 12/14/2012

    Biomedical research, and in particular cancer research, is progressing from focusing on small numbers of molecules or cellular events to global functional analysis.

  5. Optimize Bacterial Growth Studies Using Permanent Shaking And Heating 12/14/2012

    Monitoring growth of prokaryotic cells and yeast is an important tool in life science as well as in the food industry. A well-established method to quantify bacteria and yeast cells is turbidometric measurement.

  6. Why Magnetic Beads Have Several Advantages Over Direct Coupling 12/14/2012

    The use of magnetic beads as the solid phase in ELISA (Enzyme-linked immunosorbent assay) has several advantages over direct coupling to the wells. These advantages include an increased available surface area and an even distribution of beads throughout the sample providing rapid and sensitive detection of low analyte concentrations (1. Liabakk et al).

  7. Fast Quantification Of Saliva Samples In Microplates 12/7/2012

    A successful evaluation study of saliva samples in microplates using Tecan’s Infinite F50 microplate reader in combination with Tecan’s Magellan™ data analysis software, using the Saliva Quantification Kit from Greiner Bio-One for the fast detection of saliva samples.

  8. Protein A Alternatives: Mixed Mode Resins For High Throughput mAb Feedstocks 8/16/2012

    Mixed mode resins can serve as an alternative to protein A in intermediate processing. Implementation of mixed mode resins can reduce water required for filtration and time  for column loading.
     

  9. Micro-Volume Purity Assessment Of Nucleic Acids Using A260/A280 Ratio And Spectral Scanning 6/7/2012

    A common method to determine the purity of biomolecules from sample isolates is by use of a spectrophotometric ratio using absorbance measurements at wavelengths of 260 nm and 280 nm. By Peter Brescia, Applications Scientist, Applications Dept, BioTek Instruments, Inc.

  10. Chromatography Column Protection With 3M Purification LifeASSURE™ PLA Series Membrane Filters 9/14/2011
    Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.