Upstream Processing White Papers and Case Studies
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How To Analyze Nuclear Stained Cells Using A Multi-Mode Microplate Reader With DAPI-Stained Cells
4/18/2013
The imaging and analysis of fluorescently stained cells has traditionally been accomplished using manual microscopic methods with low numbers of samples, while the analysis of higher sample numbers requires the use of very expensive dedicated microscopes.
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Automate BRD4 Bromodomain TR-FRET Assays For HTS Applications
2/12/2013
Post-translational histone modifications are critical for modulation of chromatin structure by direct DNA interaction and indirect molecular interactions with recruited nuclear proteins. By Pete Brescia, Brad Larson, and Peter Banks, BioTek Instruments, Inc., and D. Bochar and L. Blazer, Cayman Chemical Co.
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Micro-Volume Determination Of Labeling Efficiency In A Standard Microplate Reader
2/12/2013
A variety of methods and reagents have been developed to efficiently label biomolecules. By Peter J. Brescia, Applications Scientist, BioTek Instruments, Inc.
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Automating A Direct, Cell-Based, Target-Compound Interaction Assay For G9a And BET Bromodomain Proteins
2/11/2013
Current biochemical assay technologies examine enzyme activity and G9a and BET bromodomain protein inhibition. Cell-based assays for these epigenetic players were previously limited to the detection of specific histone modifications using antibodies, with antibody specificity being an issue. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.
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Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells
2/10/2013
Pharmaceutical companies are increasingly exploring new biologic and biosimilar products, and thus increasing monoclonal antibody (mAb) immunotherapeutic research. By Tracy Worzella, Promega Corporation and Brad Larson, Applications Department, BioTek Instruments, Inc.
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Laser Induced Breakdown Spectroscopy (LIBS): Application Note
1/9/2013
Using a pulsed laser to create a surface ablation on a sample and then spectroscopically analyzing its elemental and molecular composition is known as Laser Induced Breakdown Spectroscopy (LIBS). This form of spectroscopy has been greatly helped with the advent of fiber optic spectrometers, as their flexibility has enabled the measurement of LIBS plasma in harsh or challenging environments. This application discussed the advantages of LIBS, and also explores different instruments that can be used in such an application.
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A Truth About Protein Detection And Analysis
12/30/2012
Whenever protein detection and analysis is referred to, many of us who are working in bioanalytical technology and method development often mistakenly believe that such detection and analysis must be quantitative.
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AlphaLISA Assays An Alternative To Conventional ELISAs
12/14/2012
AlphaLISA is a homogeneous, no-wash alternative to conventional ELISAs based on PerkinElmer’s bead-based Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology.
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Fast Magnetic Bead Purification Of Interacting Cellular Proteins
12/14/2012
Biomedical research, and in particular cancer research, is progressing from focusing on small numbers of molecules or cellular events to global functional analysis.
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Optimize Bacterial Growth Studies Using Permanent Shaking And Heating
12/14/2012
Monitoring growth of prokaryotic cells and yeast is an important tool in life science as well as in the food industry. A well-established method to quantify bacteria and yeast cells is turbidometric measurement.