Upstream Processing White Papers and Case Studies

  1. Automating A Direct, Cell-Based, Target-Compound Interaction Assay For G9a And BET Bromodomain Proteins 2/11/2013

    Current biochemical assay technologies examine enzyme activity and G9a and BET bromodomain protein inhibition. Cell-based assays for these epigenetic players were previously limited to the detection of specific histone modifications using antibodies, with antibody specificity being an issue. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  2. Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells 2/10/2013

    Pharmaceutical companies are increasingly exploring new biologic and biosimilar products, and thus increasing monoclonal antibody (mAb) immunotherapeutic research. By Tracy Worzella, Promega Corporation and Brad Larson, Applications Department, BioTek Instruments, Inc.

  3. Protein A Alternatives: Mixed Mode Resins For High Throughput mAb Feedstocks 8/16/2012

    Mixed mode resins can serve as an alternative to protein A in intermediate processing. Implementation of mixed mode resins can reduce water required for filtration and time  for column loading.
     

  4. Micro-Volume Purity Assessment Of Nucleic Acids Using A260/A280 Ratio And Spectral Scanning 6/7/2012

    A common method to determine the purity of biomolecules from sample isolates is by use of a spectrophotometric ratio using absorbance measurements at wavelengths of 260 nm and 280 nm. By Peter Brescia, Applications Scientist, Applications Dept, BioTek Instruments, Inc.

  5. Chromatography Column Protection With 3M Purification LifeASSURE™ PLA Series Membrane Filters 9/14/2011
    Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.
  6. Improved Systems For pH, DO And Conductivity Measurement In Biopharmaceutical Fermentation 9/7/2011
    When planning a modern 750 liter fermentation plant including cross flow unit, the GEA Diessel company was looking for the best available measurement technology suited for the customer requirements.
  7. Improving DNA Removal from Bioprocess Purification Processes

    Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. By 3M Purification Inc.

  8. Scale-Up Of Microbial Fermentation Using Recombinant E. Coli

    Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures.

  9. Sizing Gold Nanoparticle GNPs For Proper Conjugation

    The inertness and biocompatibility of gold nanoparticles GNPs makes them very useful for specific applications such as medical imaging, drug delivery, gene delivery, scaffold directed assembly and molecular sensing, where the size of GNPs is important. By Beckman Coulter, Inc.

  10. An Automated Profiling Application For G9a Histone Methyltransferase And Bromodomain Proteins

    Due to several findings, G9a and BET bromodomain proteins have become the target for numberous drug discovery projects. In this study, we describe a novel, cell-based assay platform that uses the robust, yet simple Enzyme Fragment Complementation (EFC) Technology.