Downstream Processing White Papers and Case Studies

1. Automating Protein Purification Processes Achieves Reliable Results And Reproducibility 10/16/2013

   ÄKTA start is an easy-to-use, reliable protein purification system. In this application note it is described how Erica, a post-doctoral researcher, used ÄKTA start to purify GST-tagged protein using a predefined method template, a GSTrap FF column, and a Frac30 fraction collector. 

2. Met One Sensors Resistant To Vaporous Hydrogen Peroxide (VHP) 1/11/2012
   Pharmaceutical manufacturers often require an aseptic production environment to minimize the presence of microbes and their potential contamination of the manufactured product.
3. Best Practices For Process Scale Column Packing Of CHT™ Ceramic Hydroxyapatite Media

   CHT ceramic hydroxyapatite media require specific considerations during process-scale chromatography packing due to high specific gravity, rapid settling rate, and sensitivity to mechanical shear.

4. Accelerating Biopharmaceutical Development From DNA To Drug 7/8/2016

   In the last decade, single-use solutions for virtually every bioprocess unit have been developed, and considerable investments have been made by the industry into areas that underpin this technology.

5. Selecting The Optimal Resins For Endotoxin Depletion In Process Purification 8/3/2016

   A guide to effectively remove endotoxins with versatile process resins.

6. Important Parameters For Packing Nuvia HR-S Resin At Process Scale 5/27/2020

   Process-scale production typically involves more than one column. The packing quality of each column affects the efficiency of the whole workflow and is an important validation criterion.

7. An Automated Profiling Application For G9a Histone Methyltransferase And Bromodomain Proteins

   Due to several findings, G9a and BET bromodomain proteins have become the target for numberous drug discovery projects. In this study, we describe a novel, cell-based assay platform that uses the robust, yet simple Enzyme Fragment Complementation (EFC) Technology.

8. Efficiencies Of Natural Convection In Passive Pallet Shippers

   Bulk transportation of temperature sensitive pharmaceuticals and biologics has become a common practice due to complex global supply chains. By Iftekhar Ahmed, Ben VanderPlas, and Stu Krupnick

9. How CHT Ceramic Hydroxyapatite Works Application Note 9/30/2016

   CHT ceramic hydroxyapatite is a versatile chromatography support used for separation of biological molecules as diverse as polyclonal and monoclonal antibodies, antibody fragments, enzymes, nucleic acids, and membrane proteins. By Paul Ng, Jie He, and Andrew Cohen, Bio-Rad Laboratories, Inc., Hercules, CA 94547

10. Guidelines For Proper Filtration And Delivery Of Samples To On-Line Process Analyzers 5/25/2012

    Increasingly precise process control strategies, often linked to computer control, have accelerated the use of sophisticated on-line chemical composition analyzers in plant applications. Instruments such as gas and liquid chromatographs, ion chromatographs, laser optic instruments, atomic absorption instruments and specific ion analyzers, which were rarities in laboratories ten years ago, are now found routinely in plant settings. By Ken Perrotta, Division Engineering Manager