Investigating The Important Parameters For Packing Nuvia HR-S Resin At Process Scale

By Xuemei He, Christopher Foster, and Payal Khandelwal, Bio-Rad Laboratories

Process-scale production typically involves more than one column. The packing quality of each column affects the efficiency of the whole workflow and is an important validation criterion. Column packing efficiency itself depends on multiple criteria. Nuvia HR-S is a strong cation exchange resin for the high resolution of protein molecules during polish purification in downstream processing. Here, we investigate the effects of packing methods, packing solutions, compression factor, resin slurry concentration, and bed height on the efficiency of packed columns. General recommendations for packing process-scale Nuvia HR-S Columns are presented.