Downstream Processing White Papers and Case Studies

  1. Micro-Volume Determination Of Labeling Efficiency In A Standard Microplate Reader 2/12/2013

    A variety of methods and reagents have been developed to efficiently label biomolecules. By Peter J. Brescia, Applications Scientist,  BioTek Instruments, Inc.

  2. Single-Use Connections Advance Aseptic Processing

    John Boehm discusses how single-use connections advance aseptic processing and create increased process flexibility and reliability while reducing costs.

  3. Improving DNA Removal from Bioprocess Purification Processes

    Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. By 3M Purification Inc.

  4. Comparison Of Five Cell Culture Media Demonstrating CHO Cell Culture Growth In Batch Or Fed-Batch Process For Commercial Operations

    This case study details a two month, four phase project to design chemically-defined feed media that improved two simulated batch processes utilizing products by SAFC, Life Technologies, Thermo Fisher Scientific, and Irvine Scientific. By Mark C. Arjona, Evan R. Horowitz, Alex A. Jurisch, Jenny Y. Bang, Tom Fletcher, Irvine Scientific

  5. Avoiding Drug Shortages With Microbial Detection 3/6/2013

    Over the past year, critical drug shortages used to treat a host of life-threatening illnesses have been making headlines. Microbial contamination has been cited as a major contributor to the shortage, yet the prevailing practices to ensure the safety of drugs have been slow to change. In most cases, quality assurance practices safeguarding the drug making environment continue to rely on periodic air and a culturing method from a prior century. By Aric Meares, President and CEO, BioVigilant

  6. Turning Data Into Information 12/22/2011

    As biopharmaceutical manufacturing has evolved from small-scale lab production in small flasks to massive commercial production in deep-tank bioreactors, an avalanche of production data has evolved to support it. From individual unit operation skids to distributed control systems and enterprise resource planning tools, biomanufacturing today is buried by the sheer quantity of data coming in from the manufacturing floor.

  7. White Paper: Computrac® Moisture Analysis Made Simple 8/10/2011
    When people aren’t feeling their best they often turn to medicine for relief from the symptoms that are associated with being ill. The public trusts that the products by pharmaceutical companies will not only make them feel better but will also not have harmful side effects.
  8. Automating Protein Purification Processes Achieves Reliable Results And Reproducibility 10/16/2013

    ÄKTA start is an easy-to-use, reliable protein purification system. In this application note it is described how Erica, a post-doctoral researcher, used ÄKTA start to purify GST-tagged protein using a predefined method template, a GSTrap FF column, and a Frac30 fraction collector. 

  9. Chromatography Column Protection With 3M Purification LifeASSURE™ PLA Series Membrane Filters 9/14/2011
    Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.
  10. Sani-Tech® Ultra White Paper 6/29/2012

    For several years, pharmaceutical manufacturing professionals have increasingly stated concerns about material leachables and extractables and their impact on the products they produce. Fluid transfer systems that include flexible tubing have been at the forefront of this discussion.