Downstream Processing White Papers and Case Studies

1. Rapid Chemically-Defined Feed Media Development to Improve Simulated Cell Culture Processes

   Implementing an optimized feed medium may be the most effective alternative for modifications to the growth medium in an existing process.

2. Examining Column Reproducibility During Method Development In Clinical Labs 6/28/2022

   In this study demonstrates how the HSS T3 stationary phase is a reliable stationary phase, as three batches made over a five-year span provide comparable results.

3. Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells 2/10/2013

   Pharmaceutical companies are increasingly exploring new biologic and biosimilar products, and thus increasing monoclonal antibody (mAb) immunotherapeutic research. By Tracy Worzella, Promega Corporation and Brad Larson, Applications Department, BioTek Instruments, Inc.

4. Materials Of Construction For Biopharmaceutical Water Systems 1/15/2013

   Stainless steels are uniquely qualified for bioprocessing applications not only because of their long service life, availability, and fabricability, but also because they are non-corroding, non-contaminant, can be polished to very smooth finishes, are strong and rigid, can withstand heat and chemical sterilization treatments, and are easily welded. By Michelle M. Gonzalez

5. Connection Technology Advances Closed System Processing

   The global demand for new biologics and vaccines, combined with the growing emergence of biosimiliars, is challenging drugmakers to re-evaluate their processes and seek ways to make them more flexible, reliable, and cost-effective. By John Boehm, Colder Products Company

6. Cultivation Of Human CAP Cells: Evaluation Of Scale-Down Capabilities 4/18/2016

   The following application note illustrates how the DASbox Mini Bioreactor System combined with the BioBLU 0.3c single-use vessels supports bioprocess development in human cell culture.

7. SciLog - Creating Advanced Biotech Equipment With Mettler Toledo 3/26/2012
   For nearly two decades, SciLog, Inc. has been dedicated to creating revolutionary single-use purification systems and disposable sensor technologies.
8. Biomanufacturing Supply Chains 12/22/2011

   This white paper examines the major issues with biopharmaceutical supply chains and how to balance inventory and risk optimally in the network.

9. Automating Protein Purification Processes Achieves Reliable Results And Reproducibility 10/16/2013

   ÄKTA start is an easy-to-use, reliable protein purification system. In this application note it is described how Erica, a post-doctoral researcher, used ÄKTA start to purify GST-tagged protein using a predefined method template, a GSTrap FF column, and a Frac30 fraction collector. 

10. Rapid Screening Of A Cell-Based Assay For GLP-1 Receptor Using A Natural Product Library 8/9/2012

    Here we demonstrate the detection of GLP-1R binding through the incorporation of a homogeneous cell-based binding assay using HTRF Tag-lite® technology. By Brad Larson and Peter Banks, BioTek Instruments, Inc. and Nicolas Pierre, Suzanne Graham, Jean-Luc Tardieu, and François Degorce, Cisbio US, Inc.