Application Note

Cultivation Of Human CAP® Cells: Evaluation Of Scale-Down Capabilities

Source: Eppendorf, Inc

By Claudia M. Hüther-Franken, Christiane Schlottbom, Helmut Kewes, and Dr. Michael Schomberg


Increasing process complexity coupled with rising cost pressures and rapidly evolving regulatory requirements makes today’s process development efforts a special challenge. The pressure of achieving faster time-to-market for new and innovative biotechnological products has led to the need to optimize every element of the total development workflow.

The following application note illustrates how the DASbox® Mini Bioreactor System combined with the BioBLU® 0.3c single-use vessels supports bioprocess development in human cell culture. Scale-down capabilities were investigated by comparison of 500˜mL cultures in a DASGIP® Parallel Bioreactor System with 170˜mL cultures in the DASbox using the BioBLU 0.3c single-use vessel.