Cell Culture White Papers & Case Studies

1. Can We Win The War Against Mycoplasma Contamination? 11/12/2015

   Mycoplasma maybe the smallest class of free living microorganism, but the consequences of a contamination in a cell batch during bioprocessing can be huge.

2. Automated Dual Feeding Into Recently Converted Bioreactors 11/2/2015

   This case study explains how Parker Bioscience Filtration (a division of Parker Hannifin) helped a pharmaceutical company optimize cell density in cell culture tanks that needed to be converted into fermenters to grow E. coli bacteria.

3. Adaption Of The BioLector Technology To Anaerobic Requirements 5/23/2014

   Biorefineries have reignited interest in anaerobic fermentations with biobutanol production being the principle driver. Already during the First World War Biobutanol and acetone were produced in Clostridium acetobutylicum.

4. Skip Steps In Cell Culturing 9/14/2013

   With Cell Culture Flask Adapters, the culture can be centrifuged directly in the flask. Data illustrates that cell yield, cell viability, and endpoint analysis results are comparable when cell cultures are processed traditionally or centrifuged directly in the flask using Cell Culture Flask Adapters.

5. Better Characterization of Biomolecules Using Agilent AdvanceBio Reversed-Phase Columns 7/2/2013

   Reversed-phase (RP) is one of the three key techniques used in biochromatography and is particularly valuable because of its compatibility with LC/MS detection. And small particle improvements, such as those found in Agilent ZORBAX RRHD 300A, 1.8 um columns, make RP an attractive choice for many biopharmaceutical applications.

6. Efficiency Test Of ReadyToProcess™ Columns 5/1/2013

   ReadyToProcess™ columns are prepacked, prequalified, and presanitized process chromatography columns suited for purification of biopharmaceuticals (e.g., proteins, vaccines, plasmids, and viruses) for clinical phase I and II studies. ReadyToProcess columns are available with several media at different volumes (1, 2.5, 10, and 20 L).

7. Automate BRD4 Bromodomain TR-FRET Assays For HTS Applications 2/12/2013

   Post-translational histone modifications are critical for modulation of chromatin structure by direct DNA interaction and indirect molecular interactions with recruited nuclear proteins. By Pete Brescia, Brad Larson, and Peter Banks, BioTek Instruments, Inc., and D. Bochar and L. Blazer, Cayman Chemical Co.

8. Automating A Direct, Cell-Based, Target-Compound Interaction Assay For G9a And BET Bromodomain Proteins 2/11/2013

   Current biochemical assay technologies examine enzyme activity and G9a and BET bromodomain protein inhibition. Cell-based assays for these epigenetic players were previously limited to the detection of specific histone modifications using antibodies, with antibody specificity being an issue. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

9. Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells 2/10/2013

   Pharmaceutical companies are increasingly exploring new biologic and biosimilar products, and thus increasing monoclonal antibody (mAb) immunotherapeutic research. By Tracy Worzella, Promega Corporation and Brad Larson, Applications Department, BioTek Instruments, Inc.

10. A Truth About Protein Detection And Analysis 12/30/2012

    Whenever protein detection and analysis is referred to, many of us who are working in bioanalytical technology and method development often mistakenly believe that such detection and analysis must be quantitative.