Insights On Polish & Viral Filtration

  1. Increasing Productivity Of pDNA DSP Using Sample Displacement Chromatography 8/27/2021

    Here, two distinct polishing processes using monoliths are compared: bind-elute (BE) and the more recently developed sample displacement purification (SDP).

  2. In-Process Control Of pDNA Production On CIMac™ pDNA Analytical Column 6/10/2021

    As the demand for plasmid DNA (pDNA) based gene therapy and vaccines increases, large-scale, cost-effective, and reproducible pDNA production is required. The key to success is a real-time in-process control method that ensures a high percentage of supercoiled pDNA in the final product. Read how the CIMac™ pDNA Analytical Column allows the monitoring of degradation products (open circular and linear pDNA), the removal of impurities (RNA), and ensures that each production step yields the amount of supercoiled pDNA anticipated.

  3. Explore Protein Function Using Surface Plasmon Resonance (SPR) 5/20/2021

    We’ve collected a few examples of how an academic research group used Biacore™ X100 SPR system in their research to investigate protein-RNA, protein-protein interactions, and how researchers have used SPR to measure antibody specificity. Download the white paper A year of interaction with Biacore™ X100.

  4. Chromatography Method For Purifying Infectious Virus Particles 5/20/2021

    This application note demonstrates a chromatography method for purifying reovirus RNA virus particles using a benchtop chromatography system, HiTrap brand columns, and Capto brand Core 700 resin.

  5. Downstream Manufacturing Of Gene Therapy Vectors 4/1/2021

    Viral vectors destined for clinical use must comply with regulatory standards for product safety and potency. Explore challenges and opportunities to improve current downstream processing operations and maximize yield.

  6. Blazar® Platform: Rodent Virus Panel From The BioReliance® Portfolio 3/12/2021

    Did you know about the alternative rapid method for in vivo MAP/HAP/RAP testing? Download our tech note Blazar® Platform: Rodent Virus Panel From The BioReliance® Portfolio to learn more about our novel degenerate PCR assay designed for the detection of a broad range of adventitious agents.

  7. Manufacturing Strategies For mRNA Vaccines And Therapeutics 2/19/2021

    mRNA has emerged as a promising modality offering a great deal of versatility for a wide range of therapeutics and vaccines with its use of non-viral delivery systems.

  8. 8 Ways Osmolality Tests Improve Cell & Gene Process Dev, Manufacturing 12/17/2020

    Emerging and novel uses for osmolality testing in advanced therapy production is helping developers address the challenges of making cell and gene therapies accessible to larger patient populations.

  9. Optimization Strategy And Process Economics Of DNA Digestion In Viral Vector Production For Gene Therapy 12/16/2020

    Benzonase® endonuclease is a high-quality product delivering immense value to viral vector processes. The enzyme activity is strongly influenced by the matrix, and optimization of its use should be a mandatory step in process development. In this application note, we highlight an optimization strategy and process economics of DNA digestion in viral vector purification.

  10. Tangential Flow Filtration (UF/DF) Of Plasmid DNA 12/16/2020

    Learn how optimization of hydraulic parameters can help you achieve the desired plasmid purity, formulation, and concentration specifications.