Downstream Isolation and Purification

  1. Effective Processes For Virus Purification 6/25/2018

    An informative presentation focused on understanding the challenges in virus/VLP purification, choosing the appropriate (or right) chromatography media for the production virus particles, and developing chromatography methods for optimal binding selectivity and recovery of virus particles.

  2. Meet The Experts: Part 1 - Process Analytical Technology (PAT) 5/1/2023

    Watch part 1 of this 4-part interview series to learn about process analytical technology (PAT) from expert Stacy Shollenberger.

  3. Optimized Purity Of A Monoclonal Antibody, Carsten Voß Video 4/12/2016

    Purity, recovery, and elution volume are important factors and parameters when screening multiple media (resins) for the development of efficient, robust, and economical production processes.

  4. Development Of A cGMP-Ready Purification Process, Carsten Voß Video 4/12/2016

    Purification results are presented of a cGMP-ready process developed for a recombinant adenovirus. The two-step column process results in an adenovirus preparation with high yield and very low host cell protein (HCP) and DNA contamination, comparable to clinical grade products.

  5. Robust Workflow For Single Cell Cloning Of iPSCs For Making cGMP Master Cell Banks 3/7/2022

    We demonstrate a platform combination of Solentim instrumentation (the VIPS™) and soluble matrix (MatriClone™, based on laminin) for the high-efficiency, high-viability single-cell cloning of iPSCs to form the Master Cell Bank.

  6. Challenges And Opportunities For Improved Chromatography Resins 6/11/2018

    Professor Giorgio Carta from the Department of Chemical Engineering at the University of Virginia gives an overview of recent advances in the development of resins for protein purification and in understanding protein-surface interactions that are responsible for separation.

  7. Virus Clearance By Chromatography 7/13/2018

    This video features a presentation of the analysis of the over all performance of different chromatography types based on their internal database looking specifically for the log reduction factors for the most testable viruses; MuLV, MVM, PRV, and Reo-3.

  8. A Scalable Immobilized Metal Affinity Chromatography Media For Process Purification 5/6/2021

    Downstream process purification of proteins requires a resin with optimized bead size for ideal pressure/flow properties and decent dynamic binding capacity DBC that provides production efficiencies and good process economics. This video explains how our newly developed metal chelate affinity resin — Nuvia IMAC — provides the mechanical strength, pore structures, ligand density, and particle size distribution required for an operation run at 300 cm/hr with a DBC of 40 mg/ml at 2 bar column backpressure.

  9. Poster Review Non–Affinity Based Purification Platform for Neutral/Basic IgMs 9/21/2018

    Review the biological properties of IgM antibodies that make them very effective vehicles for in vitro diagnostics and therapeutics.

  10. Explore Selectivity With Eshmuno® CMX Chromatography Resin 6/19/2020

    Eshmuno® CMX chromatography resin is a highly selective mixed mode chromatography resin for difficult to purify mAbs, ADCs and fusion proteins.