Cytiva product datasheets, catalogs, app notes

  1. Process For Scale-Up Of Adherent Vero Cells From Static Cell Factories To Influenza Production 5/29/2013

    In cell culture-based vaccine production, scale-up of adherent cells is challenging. This study shows a process for scaling up adherent Vero cells from static cell factories to influenza production at 50 L scale using WAVE Bioreactor™ systems and ReadyToProcess single-use equipment.

  2. Efficiency Test Of ReadyToProcess™ Columns 5/1/2013

    ReadyToProcess™ columns are prepacked, prequalified, and presanitized process chromatography columns suited for purification of biopharmaceuticals (e.g., proteins, vaccines, plasmids, and viruses) for clinical phase I and II studies. ReadyToProcess columns are available with several media at different volumes (1, 2.5, 10, and 20 L).

  3. Propagation Of Influenza Virus In Vero Cells 2/9/2012

    The aim of this work was to establish a process to produce influenza virus in Vero cell culture using single-use equipment. The end result would be a fast and simple process enabling scale-up and adaptation to industrial production. The following parameters were evaluated: medium composition, cell detachment, medium supplements, agitation conditions, and infection conditions. 

  4. Two-Step Purification Of Monoclonal IgG1 From CHO Cell Culture Supernatant 4/13/2011

    The increasing demand for monoclonal antibodies (MAb) as biopharmaceuticals has promoted the development of cell cultures with increased expression levels. As a consequence, the demand for more effi cient purifi cation processes has increased. This application note describes a two-step process for MAb purifi cation based on MabSelect SuRe™ and Capto™ adhere, a strong multimodal anion exchanger. 

  5. Screening Of Loading Conditions On Capto S Using A New High-Throughput Format, PreDictor Plates 2/13/2008

    Finding the optimal conditions for a downstream purification process is of critical importance to gain high productivity and to achieve a robust biopharmaceutical manufacturing process at large scale

  6. Automated Cross Flow Filtration For Process Development, Filter Screening, And Process Optimization At Small Scales 9/13/2007

    This application note focuses on microfiltration (pore sizes between 0.1 µm and 1 µm). Microfiltration is ideally suited for separation of cultured cells from the growth medium (broth), as well as for sterilization, and contaminant and particle removal in numerous biopharmaceutical processes

  7. Efficient, High-Titer Monoclonal Antibody Production In A Fed-Batch Process

    This application note shows the feasibility of monoclonal antibody (MAb) production in fed-batch processes using the single-use Xcellerex™ XDR-200 stirred-tank and ReadyToProcess WAVE™ 25 rocking bioreactor systems.