Application Note

Downstream Scale-Up Purification Of Influenza Virus

Source: GE Healthcare Life Sciences

This application note describes a case study where live influenza virus was purified using single-use ReadyToProcess technology. The ReadyToProcess platform is a flexible manufacturing solution, which facilitates easy handling of virus solutions and helps improve productivity. As model system for a live, attenuated influenza virus vaccine, a A/H1N1/Solomon Island strain produced in Vero cells was used. Total process time for the downstream purification was 3 days and the overall scale-up process resulted in production of influenza virus with the same yield and purity as obtained in the laboratory scale.