Upstream Processing White Papers and Case Studies

1. Accelerating Biopharmaceutical Development From DNA To Drug 7/8/2016

   In the last decade, single-use solutions for virtually every bioprocess unit have been developed, and considerable investments have been made by the industry into areas that underpin this technology.

2. Live Cell Imaging Of GPCR Dependent Second-Messenger Systems 4/15/2014

   A significant proportion of academic and drug discovery research is devoted towards the elucidation of second messenger signaling cascades resulting from interactions between G-protein coupled receptors (GPCR) and their ligands. By Paul Held Ph. D. and Peter Banks, Ph. D., BioTek Instruments, Inc. and Paul Tewson, Anne Marie Quinn and Thom Hughes, Montana Molecular

3. Semi-Automation Of A Non-Radioactive Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) Assay (Part I) 3/27/2014

   We describe the use of a non-radioactive, bioluminescent Antibody-Dependent Cell- Mediated Cytotoxicity (ADCC) assay. The protocol is a simple sequential addition of reagents and cells that can be readily automated with a simple, inexpensive pipetting station.

4. A Homogeneous Assay To Quantify Endogenous AKT Phosphorylation In Human Umbilical Vein Endothelial Cells 3/27/2014

   AKT is a serine/threonine protein kinase that plays a key role in cellular processes. AKT has become a popular target for drug discovery campaigns, due to the fact that AKT inhibitors may help to treat a number of cancers.

5. Skip Steps In Cell Culturing 9/14/2013

   With Cell Culture Flask Adapters, the culture can be centrifuged directly in the flask. Data illustrates that cell yield, cell viability, and endpoint analysis results are comparable when cell cultures are processed traditionally or centrifuged directly in the flask using Cell Culture Flask Adapters.

6. Significantly Improve FoxP3 Staining In Human Whole Blood 8/15/2013

   Regulatory T-cells (Treg) play an essential role in the control of immune responses to self and foreign antigens maintaining the balance between immunity and tolerance.

7. Better Characterization of Biomolecules Using Agilent AdvanceBio Reversed-Phase Columns 7/2/2013

   Reversed-phase (RP) is one of the three key techniques used in biochromatography and is particularly valuable because of its compatibility with LC/MS detection. And small particle improvements, such as those found in Agilent ZORBAX RRHD 300A, 1.8 um columns, make RP an attractive choice for many biopharmaceutical applications.

8. Perfusion / Cell Retention: Attaching An ATF System To Different Bioreactors 6/29/2013

   There are three methods to attach an ATF™ System to a bioreactor, depending on the type of bioreactor you use.

9. Process For Scale-Up Of Adherent Vero Cells From Static Cell Factories To Influenza Production 5/29/2013

   In cell culture-based vaccine production, scale-up of adherent cells is challenging. This study shows a process for scaling up adherent Vero cells from static cell factories to influenza production at 50 L scale using WAVE Bioreactor™ systems and ReadyToProcess single-use equipment.

10. Efficiency Test Of ReadyToProcess™ Columns 5/1/2013

    ReadyToProcess™ columns are prepacked, prequalified, and presanitized process chromatography columns suited for purification of biopharmaceuticals (e.g., proteins, vaccines, plasmids, and viruses) for clinical phase I and II studies. ReadyToProcess columns are available with several media at different volumes (1, 2.5, 10, and 20 L).