Life Science Webinars

  1. Selecting The Optimal Upstream And Downstream Intensification Strategy 5/12/2021

    To meet the demands of next-generation biomanufacturing, the industry will need multiple intensified strategies for upstream and downstream processes. Hear from Sartorius expert Priyanka Gupta as she explains how intensified solutions for upstream and downstream processes can increase productivity by 4X and reduce upfront investment by 50%.

  2. Integrated Platform For Intelligent Upstream Process Intensification 5/11/2021

    Biopharmaceutical manufacturers face pressure to increase production within their facilities while also accommodating less-stable molecules not suitable for standard fed-batch platforms. In this webinar, Sartorius expert Gerben Zijlstra walks through a product lifecycle, focusing on upstream process intensification and how PAT and perfusion-based tools are critical components of any solution.

  3. Harnessing IMAC To Enable An Immobilized Enzyme Cascade For The Synthesis Of Islatravir 5/6/2021

    In this talk, Jacob H. Forstater, Ph.D., Associate Principal Scientist, Merck Process Research & Development at Merck Research Laboratories will discuss how we utilized IMAC to enable a  streamlined through-process for the biocatalytic synthesis of islatravir, a promising nucleoside analog drug for the treatment of HIV. We discuss process design principles for the development and scale-up of robust multi-enzyme biocatalytic processes and their integration into traditional small-molecule manufacturing operations.  

  4. Two-Step Purification Using Mixed-Mode Chromatography 5/6/2021

    In this study, Daniel Yoshikawa, Ph.D., Global Product Manager at Bio-Rad Laboratories, demonstrates an efficient purification workflow for a low-expressing recombinant protein. Multiple chromatography resins were screened for the capture step of this recombinant protein with goals to minimize fermentation dilution and increase purity and recovery to greater than 75%. Optimal wash and elution conditions were determined using a design of experiment (DOE) approach. An elution buffer containing NaCl and magnesium chloride was found to purify the target molecule with high recovery and purity.

  5. Clinical Strategy For Successful Cell And Gene Therapy — How Did I Get Here? 5/3/2021

    There are major challenges to the widespread adoption of cell therapies, primarily related to the high manufacturing costs of what is largely a manual process with complex logistics and supply chain. Experts share their experiences and discuss emerging trends in the field to accelerate life-saving therapies from the bench to the bedside.

  6. Strategies For Startups — How To Structure Milestones For Successful Outcomes 5/3/2021

    Experts from the cell and gene therapy field share their experiences and discuss trends to accelerate therapies from the bench to the bedside, with specific focus on strategies for start-ups, how to look at robust scale-up, and reducing CMC risk.

  7. Attracting The Right Capital — Forming Partnerships For Long-Term Success 5/3/2021

    Experts share their experiences on investing in the cell and gene therapy field and how to form long-term partnerships for companies on the path towards bringing a potentially life-changing therapy to patients.

  8. Maximizing Product Titre By Strategic Control Of Osmolality In Mammalian Cell Bioprocesses 4/30/2021

    Osmolality is a key process parameter in mammalian cell bioprocesses that affects both specific cell growth and specific cell productivity. Published data shows that hyperosmolality, used in a targeted way at particular stages of the biological production process, for certain cell types, can enhance final product yield significantly. This presentation reviews the strategies that can be used to manipulate osmolality to increase production yields of monoclonal antibodies and other recombinant proteins.

  9. Osmolality: A Predictor In Mammalian Cell Culture 4/30/2021

    In this presentation we explored the quality and significance of osmolality as a predictor for titer, glucose consumption, and growth-inhibitory by-products such as ammonium in mammalian cell culture. The measurements of different runs were used to establish correlations with osmolality and independent runs to test the predictive power of osmolality. Interestingly the osmolality is a good predictor for glucose, ammonium, and dissolved CO2 among others with a weaker power. The implementation of osmolality measurement and its application in bioprocessing is also discussed.

  10. Upstream Applications Of Osmolality Testing In Cell & Gene Therapy Processes 4/30/2021

    Osmolality can be used as a versatile, robust tool to help improve cell and gene therapies, adding a necessary and beneficial process parameter. This presentation focuses on the incorporation of osmolality testing into upstream applications which include media development, feeding strategies, and the minimization of aggregation.