Featured Articles

91. Vaccine Production Process Development: 4 Best Practices For Scaling Up 5/14/2021

    The vaccine production process is continually refreshed by new tools and technologies which imposes challenges on process development scientists: They're the ones who have to align and optimize new methods and means with legacy products and systems. By carefully crafting a customized modular process from end to end, process development scientists can glean the benefits of new technologies without starting from scratch with each new iteration. How can researchers working on vaccine production optimize their process with customization in mind?

92. 5 Things To Consider When Customizing Cell Culture Media 5/14/2021

    Are you searching for a customized solution for your media, sera, or reagents? Here's what you should consider as you contemplate your options for bespoke lab products.

93. Hospital Researchers Turn To Corning To Help Scale Up Ex Vivo Expansion Of Adipose Stem Cells 5/13/2021

    Autologous fat grafting is the least invasive method for breast reconstruction, but also has major drawbacks, including high tissue resorption rates of up to 80%. Researchers in Denmark had a hypothesis: what if you could enrich the human fat graft with autologous adipose stem cells (ASCs) to increase graft survival? But they faced a major obstacle: how do you culture a sufficient volume of stem cells to increase grafting success? They turned to Corning, who designed a scale-up solution that’s enabling them to grow the billions of stem cells required to move forward with this clinical study.

94. First Clinical Trial Using Stem Cell Therapy To Treat Septic Shock 5/12/2021

     An Ottawa Hospital Research Institute’s Cell Manufacturing Facility successfully conducted a Phase I clinical trial examining the safety and efficacy of allogeneic bone marrow-derived MSCs as a treatment for patients experiencing septic shock. Before initiating clinical Phase II studies, the protocol for generating large quantities of MSCs had to be established to reduce end-product variability, minimize production costs, and to ensure sufficient yield to treat multiple patients. Maximizing the yield of viable cells proved challenging in a limited footprint.

95. Process Development In Gene Therapy: 4 Things To Know 5/12/2021

    Considering everything it takes to scale up gene therapy — the platforms, the media, the surfaces, the vessels, the equipment — developing viral vector production processes comes with many challenges.

96. Improving Scale-Up Efficiency 4/27/2021

    A compilation of research experiences using an industry standard for a variety of adherent cell expansion applications, including viral vector manufacturing, stem cell expansion, and vaccine production.

97. Corning® KBM581: Nutritious, Serum-free Media For CAR-T Cell Expansion 4/26/2021

    When considering an adoptive cell therapy approach, the transfusion of a sufficient number of CAR-T cells with an appropriate phenotype is critical. Corning KBM581 has been shown to be a nutritious, safe, and serum-free medium for other immune cell therapies, such as CIK and NK cells. To discover which medium among several commercialized media (including KBM581) is most appropriate for supporting CAR-T cell growth, the research group from Bioraid Bio generated CAR-T cells and analyzed the expansion folds and phenotype between different media in this application note.

98. Corning® 100 mm Aseptic Transfer Cap For 5L Erlenmeyer Flasks: An Aseptic Solution For Liquid Transfer 4/26/2021

    Closed cell culture systems play a significant role in reducing the risk of contamination during liquid handling. The Corning 100 mm aseptic transfer cap (ATC) offers a closed system solution for transferring liquid aseptically into or out of a Corning 5L Erlenmeyer flask. Here we demonstrate the use of the ATC to seed and expand two suspension cell lines, 5/9 m alpha3-18 and Sf9 cells in the 5L Erlenmeyer flasks.

99. Efficient Expansion Of HEK-293 Suspension Cell Cultures 4/26/2021

    In this study, we assessed the performance of Corning 5L Erlenmeyer flasks with different media fill volumes on the growth of HEK-293F cells, a variant of the HEK-293 cell line

100. Effective Bead-to-Bead Transfer Of Cells In Serum-free Medium Using Polystyrene Corning® Enhanced Attachment Microcarriers 4/26/2021

     To achieve large-scale cell production, a microcarrier culture scale-up strategy is necessary. Several approaches have been developed to achieve the transfer of cells from one microcarrier to another including enzymatic dissociation and bead-to-bead transfer. However, enzymatic dissociation introduces additional process steps (washing, inactivation, and removal of the enzyme) that are not feasible at a large scale. This report describes a method for expansion of anchorage-dependent cells on polystyrene Corning microcarriers with an enhanced attachment surface treatment in serum-free cell culture medium.