Removing The Aggregates Of An Acidic Monoclonal Antibody With CHT Ceramic Hydroxyapatite XT Media

By Jie He, Payal Khandelwal, and Xuemei He

CHT Ceramic Hydroxyapatite Media/Resins are known as the industry gold standard for impurity removal during monoclonal antibody (mAb) purification. In the present study, we demonstrate the removal of aggregates from the monomers of mAb S (pI ~6.9) with our newly launched CHT XT Media. mAb S was initially isolated from CHO cell culture using Protein A chromatography. The eluate from this capture step was a mixture of mAb S monomers and aggregates, with the high molecular weight species accounting for ~34% of the total mass of antibodies. Polish purification was then performed on a CHT XT Column, resulting in >96% final monomer content, thereby confirming the robustness of CHT XT in aggregate removal. In addition, excellent clearance of host cell proteins (HCPs), dsDNA, leached recombinant Protein A (rPA) and endotoxins was achieved, making CHT XT a great solution for mAb purification.