Nano Differential Scanning Calorimeter
Nano DSC is a high-resolution method for quantifying the thermal stability of large biomolecules and biotherapeutics. This instrument measures the heat of reaction from tertiary and secondary structure changes that occur when a biomolecule unfolds, or melts in the case of nucleic acids and lipids. The Nano DSC provides peak temperature when unfolding occurs and a total heat from the unfolding event.
The Nano DSC is a highly sensitivity differential scanning calorimeter for testing liquid samples containing a biomolecule or biotherapeutic sample. The unit is software controlled and experiments are set up, so that a sample is heated at a controlled rate over a defined temperature range. The Nano DSC can be automated to deliver samples directly from temperature-controlled 96 well plates, for unattended use. Automated cleaning eliminates sample carryover effects, and automated sample loading ensures the sample is delivered to the instrument the same way, every time.
The Nano DSC allows researchers to characterize the thermal stability of their samples without the use of exogenous tags or dyes, thereby simplifying workflows and reducing errors in biopharmaceutical development. Researchers can rely on the measurement reproducibility and accuracy to ensure that material changes that may occur during manufacturing or storage are easily identifiable. The Nano DSC allows researchers to test their materials under their desired conditions, without reliance on manufacturer required reagents. In one experiment, the researcher can determine the melting temperature, enthalpy and heat capacity change, allowing them to calculate the free energy to make a fully informed decision on which formulation is most stable.