Comparing Analytical Approaches For AAV Characterization

The article discusses the importance of Adeno-associated virus (AAV) sample characterization in gene therapy research, development, and manufacturing. Various analytical methods are used to assess AAV samples, including electron microscopy, analytical ultracentrifugation (AUC), charge detection mass spectrometry, quantitative polymerase chain reaction combined with enzyme-linked immunosorbent assay, size exclusion chromatography combined with multi-angle light scattering, and mass photometry. The Samux line of mass photometry products is specifically designed for AAV analysis. The choice of AAV analysis method depends on several factors, including the need to characterize multiple AAV serotypes and the ability to quantify AAV titer and detect partially filled and overfilled AAV capsids. The article suggests that mass photometry is a favorable method due to its accuracy, versatility, and low time, sample, and expertise requirements.