CHOptimizer® includes media application specialist guidance along with a portable ambr® 15 workstation that is brought onsite to optimize a CHO fed-batch process. After using CHOptimizer®, a 17 day fed-batch process was developed. From simple fed-batch (glucose only) to fed-batch, in less than 6 months an IgG1 producing cell line peak VCD was improved 2.5-fold from 4.5 × 106 cells/mL to 11 × 106 cells/mL and titer was improved 4-fold from 0.5 g/L to 1.9 g/L.
Biopharmaceutical companies today are challenged to develop high producing cell lines as quickly as possible. Commercially available media may fall short of performance expectations in order to meet targets. Alternatively, fully customized media and feed development requires significant funding, time, and in house expertise in media development. To address these challenges CHOptimizer ® offers dedicated media application specialists support, who guide customers through a series of experiments designed to optimize a fed-batch process using prequalified blends of well-characterized, Chemically Defined (CD), and Animal Origin Free (AOF) media. CHOptimizer® combines the performance of Lonza cell culture media with the power of the ambr® 15 workstation which facilitate high throughput Design of Experiments (DoE) analysis.