ZipChip: Protein Characterization And Metabolomic Analysis

Rapid protein characterization and metabolomic analysis with microfluidic technology for highly efficient separations of complex samples. Simplify sample preparation, reduce analysis time, and achieve faster results with the ZipChip CE-MS device.
ZipChip CE-MS device efficiently separates and seamlessly introduces samples into your mass spectrometer with electrospray ionization (ESI). Common challenging analyses are made easy, including protein separation for biopharmaceutical critical quality attributes (CQA), Charge Variant Analysis, and metabolite quantification from biological matrices. Experience a powerful alternative to liquid chromatography mass spectrometry (LC MS).
Rapid Analysis of Critical Quality Attributes (CQAs)
ZipChip facilitates the efficient, accurate, and comprehensive analysis of CQAs through several key features. Rapid analysis supports the development and manufacturing of high-quality products in the pharmaceutical and biopharmaceutical sectors. ZipChip facilitates CQA measurement with Charge Variant Analysis, Intact Mass, Peptide Mapping, and Reduced/Subunit analysis. The ability to rapidly and precisely analyze a wide range of samples makes ZipChip an invaluable tool to ensure product quality and safety.
High-Resolution Separation
ZipChip utilizes capillary electrophoresis (CE) integrated with mass spectrometry (MS). This combination allows for high-resolution separation of complex mixtures based on charge-to-mass ratios. It can differentiate and analyze molecules that may be challenging to distinguish using other techniques. This makes it highly effective for the assessment of critical quality attributes (CQAs) such as post-translational modifications in proteins, peptide mapping, and the analysis of small molecules.
Rapid Analysis
Significantly reduce analysis time compared to traditional methods. ZipChip can perform analyses in minutes, whereas a traditional capillary electrophoresis instrument or liquid chromatography (LC) methods might take much longer. This rapid turnaround is crucial for timely decision-making in research and manufacturing processes. The image above shows peptide mapping of the NIST mAb, with analysis done on the ZipChip in under 8 minutes. This could take hours on an LC MS system.
Minimal Sample Preparation
The ZipChip CE-MS device requires minimal sample preparation, which reduces the risk of sample loss or modification during preparation. This feature is particularly important for maintaining the integrity of the sample and ensuring accurate analysis of critical quality attributes (CQAs).
Integration with Mass Spectrometry
By integrating a capillary electrophoresis instrument with mass spectrometry, you receive detailed molecular information, including molecular weight and structural data, unlike UV-detection based capillary electrophoresis separations. This integration is invaluable for identifying and quantifying critical quality attributes (CQAs), understanding their impact on product quality, and ensuring regulatory compliance.
Versatility, Efficiency, and Cost Effectiveness
Analyze a wide range of molecules, from small ions to large biologics. ZipChip is suitable for a diverse array of applications in the pharmaceutical and biopharmaceutical industries. This versatility allows for comprehensive analysis of multiple critical quality attributes (CQAs) across different stages of product development and manufacturing. Efficiency in terms of time and resource use, coupled with minimal sample requirements, makes ZipChip a cost-effective solution for routine development and research applications.
Sample Analysis Made Easy
With the ZipChip CE-MS device, there is no stationary phase or finicky method development to slow you down. The capillary electrophoresis (CE) separates molecules based on their size and charge in an open tube. This removes matrix effects from salts, negatively charged compounds, and lipids. Most protein analyses can occur with simple dilute-and-shoot preparation, and are sensitive below a microgram per mL for Charge Variant Analysis. Amino acids and other polar metabolites can be analyzed with only protein precipitation, without the hassle of SPE, derivatization, or dry-down and reconstitution. With the ZipChip CE-MS device, you spend less time looking, and more time seeing.