Vitronectin XF™
Vitronectin XF is a recombinant, extracellular matrix (ECM) that promotes cell proliferation, maintains pluripotency, and supports normal colony morphology for MSCs and iPSCs.
The Vitronectin XF™ Advantage
Defined
Comprised of a single, defined, recombinant full-length human vitronectin sequence expressed under chemically defined conditions.
Xeno-Free
Prepared under xeno-free conditions, create a completely xeno-free system when using Vitronectin XF with chemically-defined media.
Easy to Use and Proven
Can be used at room temperature and has demonstrated ability to support normal colony morphology, proliferation, and pluripotency in iPSCs and MSCs.
Standardize Stem Cell Process Development
Take control over your culture environment with Vitronectin XF and gain more consistent cell populations and reproducible results in downstream applications.
Supports Cell Stemness and Viability in MSCs
Vitronectin XF performs equivalently to Matrigel® ECM in attributes critical to the success of MSC studies. Pluripotency as assessed by the expression of CD90, cellular morphology, as well as proliferation and viability (data not shown) are equivalent to the murine-based Matrigel ECM. As a defined, xeno-free ECM, Vitronectin XF is a superior choice to streamline regulatory hurdles and consistency in your development.
Maintains Pluripotency in iPSCs
Cryopreserved human induced pluripotent stem cells (hiPSCs) were thawed and recovered, then split on Vitronectin XF plates. After 5 passages, the cells were subjected to flow cytometry testing for the pluripotency markers Oct4 (yellow curve), SSEA4 (not shown), and Tra1-81 (not shown). The green curve indicates negative control cells.
Maintains Normal Colony Morphology in iPSCs
Morphological characteristics play a critical role in indicating pluripotency in iPSCs, and in determining the time of passaging in both iPSC and MSC assays. Vitronectin XF maintains normal colony morphology for iPSCs comparably to Matrigel®.