Use Of Roche Recombinant Trypsin For Cell Culture Applications

By Dr. Andrea Normann, Dr. Manuela Poignee-Heger, and Dr. Peter Hloch

Recombinant trypsin offers a safer alternative to traditional cell dissociation methods. At just 7 μg/ml—substantially lower than conventional 0.25% solutions—this animal-free enzyme achieves equivalent cell detachment across sensitive and robust cell lines while minimizing potential damage. Performance data from MRC-5, Vero, CHO, MDCK, and stem cell lines demonstrate 88-98% viability with detachment times of 1.5-7 minutes. The formulation maintains activity for 36 months at -20°C and supports consistent growth through multiple passages.

For vaccine production and biopharmaceutical manufacturing requiring GMP-compliant, high-purity reagents with reduced cellular stress, this optimized concentration provides reliable results without compromising cell health or productivity.