Understanding Nanodifferential Scanning Fluorometry (nanoDSF) Data And Which Variable To Design Your Experiment Around

In this presentation, Stefanie Kall, PhD, and Bridget Milorey, PhD, discuss the use of nanodifferential scanning fluorometry (nanoDSF) to assess the conformational stability of proteins. They explain how nanoDSF data is generated and highlight the significance of parameters such as the melting temperature (TM) and the onset of melting (T onset) in determining protein stability.

They also discuss the application of nanoDSF to monoclonal antibodies and antibody-drug conjugates (ADCs) in biologics development. They explain that ADCs are used to target cancer cells but can potentially destabilize the antibody. Factors that can impact ADC development include the conjugation process, the drug itself, and buffer conditions. The speakers emphasize the need for high-resolution fingerprint data in addition to TM and TON parameters. Overall, the presentation emphasizes the importance of evaluating multiple parameters and considering all aspects of stability when assessing biologics. The speakers stress the need for careful planning, consistent sample handling, and proper experimental design to obtain quality data.