Transitioning From 2D To 3D Cell Culture: Getting Started With Spheroids

The need to move beyond traditional 2D cell culture is becoming increasingly urgent, as regulatory bodies like the FDA and EMA now encourage or require more physiologically relevant models in drug submissions. LICORbio’s Jessica Zinskie, PhD, provides a practical framework for getting started with spheroids, from understanding different 3D models and selecting the best ultra-low adhesion plate to optimizing cell seeding density and choosing 3D-validated reagents. She also introduces the LICORbio Atlas™ Imager and 3D Essentials Kit to streamline your protocol development and provide built-in quality control at every step.

Whether you're new to 3D models or looking to refine an existing workflow, you'll learn how to move beyond 2D cell culture to develop reproducible spheroid cultures and generate biologically meaningful 3D data.