Suspension Culture On Microcarriers And As Aggregates Enables Expansion And Differentiation Of Pluripotent Stem Cells

Human pluripotent stem cells (PSCs) hold great promise for regenerative therapies due to their ability to differentiate into various mature cell types. Traditional 2D culture methods cannot meet the demand for large-scale cell production required for clinical applications. This application note explores the use of bioreactors and 3D culture strategies for efficient PSC expansion.

Key Topics Covered:

• PSC Morphology and Cell Cycle: PSCs grown on microcarriers or as aggregates in suspension culture retain typical PSC cell cycle profiles and morphology.
• Differentiation Potential: PSCs cultured in 3D suspension conditions maintain their pluripotency and differentiation potential into various lineages.
• Neural Progenitor Cells and Cardiomyocytes: PSCs expanded in 3D suspension cultures can be effectively differentiated into neural progenitor cells and cardiomyocytes, both of which have significant clinical relevance.

Access the full content to learn more about the advantages and implications of using bioreactors for large-scale PSC expansion.