Supporting Cells To Overcome Shear Forces While Maintaining An Animal Component Free Workflow

Expanding clones from static to shaking conditions during the development of cell lines can be quite a challenge. While Foetal Bovine Serum (FBS) is well established in its ability to support cells overcome shear forces, its application raises regulatory concerns due to increased risk of contamination of health products, and batch variability.

How do we support the expansion of cells to shaking vessels to accelerate the development of new protein therapeutics with FBS out of the picture? InstiSHAKE™ CHO is an animal component free cell culture supplement, specifically designed to support cells adapting to shaking culture conditions. The product is simply added to the basal culture medium on the day of expansion to support the viability and viable cell density of cells.