Stressing Cells: The Role Of Cryoprotectants In ATMP Cryopreservation

By Khalil Essani, Product Line Management

Cell and tissue therapies require a sufficient number of viable, high functioning cells, which can be achieved through cryopreservation. This process of freezing cells preserves their viability and functionality, providing a longer shelf life and greater flexibility for patients and manufacturing processes. However, cells must survive extreme conditions such as ice crystal formation and exposure to cell toxic cryoprotectants. Cryoprotective agents (CPAs) are used to reduce the effects of these conditions. Both permeating and non-permeating CPAs have their advantages and disadvantages, with the former being more effective but also more toxic. There is a need for future stability studies, substitution of toxic CPAs with less toxic ones, and implementation of safe tracking and data security systems in the supply chain.